Osteoprotegerin Kit ELISA
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- Antigène Voir toutes Osteoprotegerin (TNFRSF11B) Kits ELISA
- Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))
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Reactivité
- Porc
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.1-30 ng/mL
- Seuil minimal de détection
- 0.1 ng/mL
- Application
- ELISA
- Fonction
- Porcine Osteoprotegerin (TNFRSF11B) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- The antibody pair provided in this kit recognizes Porcine Osteoprotegerin (TNFRSF11B)
- Sensibilité
- 0.12 ng/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Procédure de l'essai
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes Osteoprotegerin (TNFRSF11B) Kits ELISA
- Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))
- Autre désignation
- Osteoprotegerin (TNFRSF11B Produits)
- Synonymes
- CD254 Kit ELISA, ODF Kit ELISA, OPGL Kit ELISA, OPTB2 Kit ELISA, RANKL Kit ELISA, TRANCE Kit ELISA, hRANKL2 Kit ELISA, sOdf Kit ELISA, OCIF Kit ELISA, OPG Kit ELISA, TR1 Kit ELISA, Opg Kit ELISA, ocif Kit ELISA, opg Kit ELISA, tr1 Kit ELISA, TNF superfamily member 11 Kit ELISA, TNF receptor superfamily member 11b Kit ELISA, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) Kit ELISA, TNF receptor superfamily member 11B Kit ELISA, tumor necrosis factor receptor superfamily member 11b S homeolog Kit ELISA, tumor necrosis factor receptor superfamily, member 11b Kit ELISA, TNFSF11 Kit ELISA, TNFRSF11B Kit ELISA, Tnfrsf11b Kit ELISA, tnfrsf11b.S Kit ELISA
- Classe de substances
- Chemical
- Sujet
- Osteoprotegerin (TNFRSF11B)
- ID gène
- 100738370
- UniProt
- F1S278
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