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PAI1 Kit ELISA

SERPINE1 Reactivité: Humain, Souris Colorimetric Sandwich ELISA 80-20.000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2748503
  • Antigène Voir toutes PAI1 (SERPINE1) Kits ELISA
    PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
    Reactivité
    • 10
    • 7
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    80-20.000 pg/mL
    Seuil minimal de détection
    80 pg/mL
    Application
    ELISA
    Fonction
    Mouse PAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects mouse and human PAI-1.
    Sensibilité
    80 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples20 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes PAI1 (SERPINE1) Kits ELISA
    PAI1 (SERPINE1) (Plasminogen Activator Inhibitor 1 (SERPINE1))
    Autre désignation
    PAI-1 (SERPINE1 Produits)
    Synonymes
    PAI Kit ELISA, PAI-1 Kit ELISA, PAI1 Kit ELISA, PLANH1 Kit ELISA, SERPINE1 Kit ELISA, si:ch211-138a11.1 Kit ELISA, Planh1 Kit ELISA, PAI1A Kit ELISA, Pai1 Kit ELISA, Pai1aa Kit ELISA, Planh Kit ELISA, RATPAI1A Kit ELISA, serpin family E member 1 Kit ELISA, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog Kit ELISA, serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade E, member 1 Kit ELISA, SERPINE1 Kit ELISA, serpine1.L Kit ELISA, serpine1 Kit ELISA, Serpine1 Kit ELISA
    Sujet
    Plasminogen activator inhibitor 1 (PAI) (PAI-1) (Endothelial plasminogen activator inhibitor) (Serpin E1)
    UniProt
    P22777
    Pathways
    Signalisation p53, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
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