SERPINE1 Kit ELISA (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1)

Details for Product SERPINE1 ELISA Kit No. ABIN2748504, Fournisseur: Connectez-vous pour afficher
Antigène
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • SERPINE1
  • si:ch211-138a11.1
  • Planh1
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin family E member 1
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 L homeolog
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • SERPINE1
  • serpine1.L
  • serpine1
  • Serpine1
Reactivité
Humain, Souris, Macaque rhésus
Alternatives
Kits with alternative reactivity to:
38
23
20
14
11
7
3
3
3
2
2
1
1
Type de méthode
Sandwich ELISA
Gamme de detection
20-6.000 pg/mL
Seuil minimal de détection
20 pg/mL
Application
ELISA
Options
Fournisseur
Connectez-vous pour afficher
N° du produit (Fournisseur)
Connectez-vous pour afficher
Fonction Rhesus Monkey PAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon Plasma, Cell Culture Supernatant, Serum
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This ELISA antibody pair detects Rhesus Monkey, mouse and human PAI-1.
Sensibilité 20 pg/mL
Attributs du produit
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Autre désignation PAI-1 (SERPINE1 ELISA Kit Extrait)
Sujet Plasminogen activator inhibitor-1
ID gène 713883
UniProt F6Y5D7
Pathways Signalisation p53, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
Indications d'application Recommended Dilution for serum and plasma samples100 fold
Volume d'échantillon 100 μL
Plaque Pre-coated
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Procédure de l'essai

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Restrictions For Research Use only
Stock -20 °C
Stockage commentaire The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption 6 months
Images (Fournisseur)
ELISA image for serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kit (ABIN2748504) serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kit
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