RPS6 Kit ELISA

N° du produit ABIN2748619

Détail du produit RPS6 Kit ELISA

Antigène: RPS6 (Ribosomal Protein S6 (RPS6))

Épitope: pSer235, pSer236, total

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human/Mouse/Rat Phospho-RPS6 (S235/S236) and Total RPS6 ELISA Kit. This assay semi-quantitatively measures phophorylated RPS6 (Ser235/S236) and Total RPS6 in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes human, mouse, or rat Phospho-RPS6 (pSer235/Ser236) and total RPS6.

Attributs du produit:

• Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Procédure de l'essai:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: RPS6 (Ribosomal Protein S6 (RPS6))

Autre désignation: RPS6 (RPS6 Produits)

Synonymes: S6 Kit ELISA, rps6 Kit ELISA, rps6a Kit ELISA, S6R Kit ELISA, (Rp)S6 Kit ELISA, CG10944 Kit ELISA, DS6 Kit ELISA, Dmel\\CG10944 Kit ELISA, M(1)7BC Kit ELISA, M(1)7C Kit ELISA, RPS6 Kit ELISA, Rp S6 Kit ELISA, Rps6 Kit ELISA, air8 Kit ELISA, air[8] Kit ELISA, anon-WO02059370.61 Kit ELISA, hen Kit ELISA, l(1)air8 Kit ELISA, l(1)air[8] Kit ELISA, l(1)hen Kit ELISA, pp30 Kit ELISA, rpS6 Kit ELISA, wu:fa92e06 Kit ELISA, wu:fb64g06 Kit ELISA, zgc:92237 Kit ELISA, ribosomal protein S6 Kit ELISA, ribosomal protein S6 S homeolog Kit ELISA, S6 ribosomal protein Kit ELISA, Ribosomal protein S6 Kit ELISA, 30S ribosomal protein S6 Kit ELISA, 40S ribosomal protein S6 Kit ELISA, RPS6 Kit ELISA, rps6.S Kit ELISA, LOC100135859 Kit ELISA, Rps6 Kit ELISA, RpS6 Kit ELISA, rps6 Kit ELISA, rps-6 Kit ELISA, LOC100533219 Kit ELISA

Sujet: Ribosomal protein S6 phosphorylated at S235/S236 and total RPS6

ID gène: 6194

UniProt: P62753

Pathways: Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly