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FLT1 Kit ELISA

FLT1 Reactivité: Rat Colorimetric Sandwich ELISA 0.41 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2748745
  • Antigène Voir toutes FLT1 Kits ELISA
    FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
    Reactivité
    • 6
    • 6
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.41 ng/mL
    Seuil minimal de détection
    0.41 ng/mL
    Application
    ELISA
    Fonction
    Rat VEGFR1 ELISA Kit for serum, plasma, and cell culture supernatant samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects Rat VEGFR1 and Mouse VEGFR1. Other species not determined.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes FLT1 Kits ELISA
    FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))
    Autre désignation
    VEGFR1 (FLT1 Produits)
    Synonymes
    FLT Kit ELISA, FLT-1 Kit ELISA, VEGFR-1 Kit ELISA, VEGFR1 Kit ELISA, FLT1 Kit ELISA, flt1b Kit ELISA, sFlt1 Kit ELISA, AI323757 Kit ELISA, Flt-1 Kit ELISA, fms related tyrosine kinase 1 Kit ELISA, FMS-related tyrosine kinase 1 Kit ELISA, vascular endothelial growth factor receptor 1 Kit ELISA, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Kit ELISA, FMS-like tyrosine kinase 1 Kit ELISA, vascular endothelial growth factor receptor-1 Kit ELISA, FLT1 Kit ELISA, Flt1 Kit ELISA, CpipJ_CPIJ012087 Kit ELISA, flt1 Kit ELISA, FLT-1 Kit ELISA
    Sujet
    Vascular Endothelial Growth Factor Receptor 1 (Flt1)
    ID gène
    54251
    UniProt
    P53767
    Pathways
    Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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