ADAMTS4 Kit ELISA

N° du produit ABIN2859239

Détail du produit ADAMTS4 Kit ELISA

Antigène: ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))

Épitope: AA 213-685

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625-40.000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAMTS4

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: F213-C685

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <20pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: CHO
Immunogen sequence: F213-C685

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 disintegrin domain.

Tissue Specificity: Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles. Isoform 2 is detected in osteoarthritic synovium. .

Plaque: Pre-coated

Protocole: human ADAMTS4 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been precoated onto 96-well plates. Standards(CHO, F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS4 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 40,000pg/mL, 20,000pg/mL, 10,000pg/mL, 5,000pg/mL, 2,500pg/mL, 1,250pg/mL, 625pg/mL human ADAMTS4 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAMTS4 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 5.9, Standard deviation: 0.248, CV(%): 4.2
• Sample 2: n=16, Mean(ng/ml): 12, Standard deviation: 0.612, CV(%): 5.1
• Sample 3: n=16, Mean(ng/ml): 21, Standard deviation: 1.2, CV(%): 5.7,
• Sample 1: n=24, Mean(ng/ml): 6.5, Standard deviation: 0.377, CV(%): 5.8
• Sample 2: n=24, Mean(ng/ml): 14, Standard deviation: 0.882, CV(%): 6.3
• Sample 3: n=24, Mean(ng/ml): 23, Standard deviation: 1.66, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: ADAMTS4 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 4 (ADAMTS4))

Autre désignation: ADAMTS4 (ADAMTS4 Produits)

Synonymes: 3830423K05 Kit ELISA, ADAM-TS4 Kit ELISA, ADAMTS-2 Kit ELISA, ADMP-1 Kit ELISA, mKIAA0688 Kit ELISA, ADAMTS-4 Kit ELISA, ADAMTS4 Kit ELISA, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 4 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif, 4 Kit ELISA, Adamts4 Kit ELISA, ADAMTS4 Kit ELISA

Sujet:

Protein Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-|-Ala-393' site.

Background: ADAMTS4, A disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 4,ADAM-TS 4,ADAM-TS4,ADAMTS-4,3.4.24.82,ADMP-1,Aggrecanase-1,ADAMTS4,KIAA0688,UNQ769/PRO1563,

Full Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 4

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 9507

UniProt: O75173