COL18A1 Kit ELISA

N° du produit ABIN2859242

Détail du produit COL18A1 Kit ELISA

Antigène: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Épitope: AA 1591-1774

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10.000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Endostatin

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: H1591-K1774

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: H1591-K1774

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the multiplexin collagen family.

Tissue Specificity: Expressed in liver, kidney, lung, skeletal muscle and testis. .

Plaque: Pre-coated

Protocole: mouse Endostatin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Endostatin has been precoated onto 96-well plates. Standards(NSO, H1591-K1774) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Endostatin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Endostatin amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse Endostatin standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(ng/ml): 0.8, Standard deviation: 0.052, CV(%): 6.5
• Sample 2: n=16, Mean(ng/ml): 2.14, Standard deviation: 0.135, CV(%): 6.3
• Sample 3: n=16, Mean(ng/ml): 4.25, Standard deviation: 0.179, CV(%): 4.2,
• Sample 1: n=24, Mean(ng/ml): 0.84, Standard deviation: 0.06, CV(%): 7.1
• Sample 2: n=24, Mean(ng/ml): 2.23, Standard deviation: 0.147, CV(%): 6.6
• Sample 3: n=24, Mean(ng/ml): 4.37, Standard deviation: 0.253, CV(%): 5.8

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour COL18A1 Kit ELISA (ABIN2859242)

Guo, Wu, Cen, Li, Kong, Zhou: "IL-22-producing Th22 cells play a protective role in CVB3-induced chronic myocarditis and dilated cardiomyopathy by inhibiting myocardial fibrosis." dans: Virology journal, Vol. 11, Issue 1, pp. 2511, (2014) (PubMed).

Fu, Zhao, Wu, Wu, Xu, Liu, Tong: "Therapeutic mechanisms of Tongmai Dasheng Tablet on tripterygium glycosides induced rat model for premature ovarian failure." dans: Journal of ethnopharmacology, Vol. 139, Issue 1, pp. 26-33, (2011) (PubMed).

Zhang, Qu, Cui, Guo, Zhang, Ma, Sun: "Combined endostatin and TRAIL gene transfer suppresses human hepatocellular carcinoma growth and angiogenesis in nude mice." dans: Cancer biology & therapy, Vol. 8, Issue 5, pp. 466-73, (2009) (PubMed).

Détail du antigène

Antigène: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Autre désignation: Endostatin (COL18A1 Produits)

Synonymes: KNO Kit ELISA, KNO1 Kit ELISA, KS Kit ELISA, cb371 Kit ELISA, cb529 Kit ELISA, fc96d06 Kit ELISA, sb:cb529 Kit ELISA, wu:fb47h10 Kit ELISA, wu:fb77g12 Kit ELISA, wu:fc50f04 Kit ELISA, wu:fc96d06 Kit ELISA, wu:fk02h12 Kit ELISA, kno Kit ELISA, kno1 Kit ELISA, collagen type XVIII alpha 1 chain Kit ELISA, collagen type XVIII alpha 1 chain a Kit ELISA, collagen, type XVIII, alpha 1 Kit ELISA, collagen type XVIII alpha 1 Kit ELISA, COL18A1 Kit ELISA, col18a1a Kit ELISA, Col18a1 Kit ELISA, col18a1 Kit ELISA

Classe de substances: Chemical

Sujet:

Protein Function: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And It is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Synonyms: Collagen alpha-1(XVIII) chain,Endostatin,Col18a1,

Full Gene Name: Collagen alpha-1(XVIII) chain

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 12822

UniProt: P39061