HGF Kit ELISA

N° du produit ABIN2859322

Détail du produit HGF Kit ELISA

Antigène: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Épitope: AA 33-495, AA 496-728

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse HGF

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: Q33-R495 (i±)&V496-L728 (i²)

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: Q33-R495 (alpha)&V496-L728 (beta)

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the peptidase S1 family. Plasminogen subfamily.

Plaque: Pre-coated

Protocole: mouse HGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for HGF has been precoated onto 96-well plates. Standards(NSO,Q33-R495 (α) & V496 - L728 (β)) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse HGF amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse HGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse HGF standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 427, Standard deviation: 17.93, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1523, Standard deviation: 76.15, CV(%): 5.0
• Sample 3: n=16, Mean(pg/ml): 3342, Standard deviation: 187.15, CV(%): 5.6,
• Sample 1: n=24, Mean(pg/ml): 501, Standard deviation: 37.58, CV(%): 7.5
• Sample 2: n=24, Mean(pg/ml): 1756, Standard deviation: 136.97, CV(%): 7.8
• Sample 3: n=24, Mean(pg/ml): 3471, Standard deviation: 284.62, CV(%): 8.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour HGF Kit ELISA (ABIN2859322)

Secchiero, Corallini, Zavan, Tripodo, Vindigni, Zauli: "Mesenchymal stem cells display hepato-protective activity in lymphoma bearing xenografts." dans: Investigational new drugs, Vol. 30, Issue 2, pp. 803-7, (2012) (PubMed).

Détail du antigène

Antigène: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Autre désignation: HGF (HGF Produits)

Synonymes: DFNB39 Kit ELISA, F-TCF Kit ELISA, HGFB Kit ELISA, HPTA Kit ELISA, SF Kit ELISA, HGF Kit ELISA, C230052L06Rik Kit ELISA, HGF/SF Kit ELISA, NK1 Kit ELISA, NK2 Kit ELISA, SF/HGF Kit ELISA, hepatocyte growth factor Kit ELISA, HGF Kit ELISA, Hgf Kit ELISA

Sujet:

Protein Function: Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. .

Background: Hepatocyte growth factor(HGF) is the most potent mitogen for mature parenchymal hepatocytes in primary culture, and seems to be a hepatotrophic factor that acts as a trigger for liver regeneration after partial hepatectomy and liver injury. HGF has a relative molecular mass(Mr) of 82,000 and is a heterodimer composed of a large alpha-subunit of Mr 69,000 and a small beta-subunit of Mr 34,000. The protein consists of 728 amino acid residues, including a possible signal peptide at the N-terminus. HGF may serve as a paracrine mediator to control placental development and growth. This growth factor may play an important role as a paracrine mediator of the proliferation of melanocytes and endothelial cells, as well as cells of epithelial origin. The gene encoding the human HGF is assigned to human chromosome 7.

Synonyms: Hepatocyte growth factor,Hepatopoietin-A,Scatter factor,SF,Hepatocyte growth factor alpha chain,Hepatocyte growth factor beta chain,Hgf,

Full Gene Name: Hepatocyte growth factor

ID gène: 15234

UniProt: Q08048

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor