FCGR2B Kit ELISA

N° du produit ABIN2859328

Détail du produit FCGR2B Kit ELISA

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Épitope: AA 40-217

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2-2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse CD32/FCGR2b/c

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: T40-R217

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: T40-R217

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Tissue Specificity: Widely expressed by cells of hemopoietic origin. The isoforms are differentially expressed. Isoform IIB1 is preferentially expressed by cells of the lymphoid lineage, isoform IIB2 by cells of the myeloid lineage, and isoform IIB3 is released by macrophages and is present in the serum. Isoform IIB1' is expressed in myeloid and lymphoid cell lines, in normal spleen cells, and in resting or LPS-activated B-cells but is not detected in mesenteric lymph node cells.

Plaque: Pre-coated

Protocole: mouse CD32 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD32 has been precoated onto 96-well plates. Standards(NSO, T40-R217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD32 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD32 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse CD32 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD32 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 195, Standard deviation: 8.19, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 472, Standard deviation: 22.66, CV(%): 4.8
• Sample 3: n=16, Mean(pg/ml): 1023, Standard deviation: 521.7, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 216, Standard deviation: 11.88, CV(%): 5.5
• Sample 2: n=24, Mean(pg/ml): 515, Standard deviation: 31.93, CV(%): 6.2
• Sample 3: n=24, Mean(pg/ml): 1174, Standard deviation: 81, CV(%): 6.9

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Détail du antigène

Antigène: FCGR2B (Fc Fragment of IgG, Low Affinity IIb, Receptor (CD32) (FCGR2B))

Autre désignation: FCGR2B (FCGR2B Produits)

Synonymes: CD32 Kit ELISA, CD32B Kit ELISA, FCG2 Kit ELISA, FCGR2 Kit ELISA, IGFR2 Kit ELISA, FCGR2B Kit ELISA, AI528646 Kit ELISA, F630109E10Rik Kit ELISA, Fc[g]RII Kit ELISA, FcgRII Kit ELISA, Fcgr2 Kit ELISA, Fcgr2a Kit ELISA, Fcr-2 Kit ELISA, Fcr-3 Kit ELISA, Ly-17 Kit ELISA, Ly-m20 Kit ELISA, LyM-1 Kit ELISA, Lym-1 Kit ELISA, FCRII Kit ELISA, FcRII Kit ELISA, Fc fragment of IgG receptor IIb Kit ELISA, Fc fragment of IgG, low affinity IIb, receptor (CD32) Kit ELISA, Fc receptor, IgG, low affinity IIb Kit ELISA, low affinity immunoglobulin gamma Fc region receptor II Kit ELISA, FCGR2B Kit ELISA, Fcgr2b Kit ELISA, LOC100856270 Kit ELISA

Sujet:

Protein Function: Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of antigen-antibody complexes from the circulation and modulation of antibody production by B-cells. Isoform IIB1 and isoform IIB1' form caps but fail to mediate endocytosis or phagocytosis. Isoform IIB2 can mediate the endocytosis of soluble immune complexes via clathrin- coated pits. Isoform IIB1 and isoform IIB2 can down-regulate B- cell, T-cell, and mast cell activation when coaggregated to B-cell receptors for AG (BCR), T-cell receptors for AG (TCR), and Fc receptors, respectively.

Background: CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Synonyms: Low affinity immunoglobulin gamma Fc region receptor II,Fc gamma receptor IIB,Fc-gamma RII,Fc-gamma-RIIB,FcRII,IgG Fc receptor II beta,Lymphocyte antigen 17,Ly-17,CD32,Fcgr2,Fcgr2b, Ly-17,

Full Gene Name: Low affinity immunoglobulin gamma Fc region receptor II

Cellular Localisation: Cell membrane, Single-pass type I membrane protein.

ID gène: 14130

UniProt: P08101

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, BCR Signaling