ADAMTS1 Kit ELISA

N° du produit ABIN2859342

Détail du produit ADAMTS1 Kit ELISA

Antigène: ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))

Épitope: AA 253-734

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 93.8-6000 pg/mL

Seuil minimal de détection: 93.8 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ADAMTS1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin)

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO
Immunogen sequence: F253-A734

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <10pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: NSO
Immunogen sequence: F253-A734

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Contains 1 disintegrin domain.

Plaque: Pre-coated

Protocole: human ADAMTS1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been precoated onto 96-well plates. Standards(NSO, F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m human ADAMTS1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human ADAMTS1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 1208, Standard deviation: 42.28, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 2741, Standard deviation: 117.9, CV(%): 4.3
• Sample 3: n=16, Mean(pg/ml): 4137, Standard deviation: 202.7, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 1850, Standard deviation: 98.05, CV(%): 5.3
• Sample 2: n=24, Mean(pg/ml): 3035, Standard deviation: 176.03, CV(%): 5.8
• Sample 3: n=24, Mean(pg/ml): 4726, Standard deviation: 302.5, CV(%): 6.4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour ADAMTS1 Kit ELISA (ABIN2859342)

Tang, Lee, Kim, Bishop, Rodgers: "siRNA-knockdown of ADAMTS-13 modulates endothelial cell angiogenesis." dans: Microvascular research, Vol. 113, pp. 65-70, (2018) (PubMed).

Détail du antigène

Antigène: ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))

Autre désignation: ADAMTS1 (ADAMTS1 Produits)

Synonymes: c3-c5 Kit ELISA, meth1 Kit ELISA, xadamts1 Kit ELISA, C3-C5 Kit ELISA, METH1 Kit ELISA, ADAM-TS1 Kit ELISA, ADAMTS Kit ELISA, ADAMTS-1 Kit ELISA, METH-1 Kit ELISA, adamts1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif, 1 Kit ELISA, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 1 L homeolog Kit ELISA, ADAMTS1 Kit ELISA, adamts1 Kit ELISA, Adamts1 Kit ELISA, adamts1.L Kit ELISA

Sujet:

Protein Function: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. .

Background: ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.

Synonyms: A disintegrin and metalloproteinase with thrombospondin motifs 1,ADAM-TS 1,ADAM-TS1,ADAMTS-1,3.4.24.-,METH-1,ADAMTS1,KIAA1346, METH1,

Full Gene Name: A disintegrin and metalloproteinase with thrombospondin motifs 1

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

ID gène: 9510

UniProt: Q9UHI8

Pathways: SARS-CoV-2 Protein Interactome