c-MYC Kit ELISA

N° du produit ABIN2866260

Détail du produit c-MYC Kit ELISA

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marque: TransAM®

Type d'échantillon: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificité: TransAM c-Myc Kits are tested for sensitivity in detecting c-Myc activation.

Attributs du produit: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Ingrédients: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Nuclear extracts prepared from unstimulated Jurkat (1 day growth) and U-937 cells are diluted to 0.625 μg/well and assayed using the TransAM c-Myc Kit. The ratio of the signals from the Jurkat cells over the U-937 cells must be above 4. Lot No. 06012008 was developed for 2.5 minutes. It gave a ratio of 403.5 (Figure 1). The endogenous level of c-Myc expression, and this ratio may vary depending on the cell type tested and the treatment used. TransAM c-Myc Kits are also tested for specificity in detecting c-Myc activity. TransAM c-Myc assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated c-Myc consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents c-Myc binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has no effect on c-Myc binding.

Durée du test: 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, primary antibody, DTT and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.

Date de péremption: 6 months

Détail du antigène

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: C-Myc (MYC Produits)

Synonymes: MRTL Kit ELISA, MYCC Kit ELISA, bHLHe39 Kit ELISA, c-Myc Kit ELISA, CMYC Kit ELISA, c-myc Kit ELISA, C-MYC Kit ELISA, LOC100136746 Kit ELISA, ARC Kit ELISA, B430311C09Rik Kit ELISA, MYC Kit ELISA, NOP Kit ELISA, Nop30 Kit ELISA, RNCMYC Kit ELISA, mMyc Kit ELISA, AU016757 Kit ELISA, Myc2 Kit ELISA, Niard Kit ELISA, Nird Kit ELISA, cmyc Kit ELISA, zc-myc Kit ELISA, c-myc II Kit ELISA, myc-B Kit ELISA, myc2 Kit ELISA, myc1 Kit ELISA, CMyc Kit ELISA, zgc:55680 Kit ELISA, CG10798 Kit ELISA, D-Myc Kit ELISA, DM Kit ELISA, DMYc Kit ELISA, Dm Kit ELISA, Dmel\CG10798 Kit ELISA, Dmyc Kit ELISA, EG:BACN5I9.1 Kit ELISA, anon-WO03040301.171 Kit ELISA, bHLHe57 Kit ELISA, c-MYC Kit ELISA, d-myc Kit ELISA, dMYC Kit ELISA, dMyc Kit ELISA, dMyc1 Kit ELISA, da Kit ELISA, dm Kit ELISA, dm/dMyc Kit ELISA, dm/myc Kit ELISA, dmyc Kit ELISA, dmyc1 Kit ELISA, l(1)G0139 Kit ELISA, l(1)G0354 Kit ELISA, l(1)G0359 Kit ELISA, myc Kit ELISA, MYC proto-oncogene, bHLH transcription factor Kit ELISA, v-myc myelocytomatosis viral oncogene homolog Kit ELISA, nucleolar protein 3 (apoptosis repressor with CARD domain) Kit ELISA, myelocytomatosis oncogene Kit ELISA, MYC proto-oncogene, bHLH transcription factor a Kit ELISA, MYC proto-oncogene, bHLH transcription factor L homeolog Kit ELISA, MYC proto-oncogene, bHLH transcription factor S homeolog Kit ELISA, v-myc myelocytomatosis viral oncogene homolog (avian) Kit ELISA, MYC proto-oncogene, bHLH transcription factor b Kit ELISA, CG10798 gene product from transcript CG10798-RB Kit ELISA, MYC Kit ELISA, LOC100136746 Kit ELISA, Nol3 Kit ELISA, Myc Kit ELISA, myca Kit ELISA, myc.L Kit ELISA, myc.S Kit ELISA, mycb Kit ELISA

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg