NFATC1 Kit ELISA

N° du produit ABIN2866278

Détail du produit NFATC1 Kit ELISA

Antigène: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: DNA-Binding ELISA

Application: ELISA

Fonction: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marque: TransAM®

Type d'échantillon: Cell Extracts, Tissue Samples

Analytical Method: Quantitative

Specificité: TransAM NFATc1 Kits are tested for sensitivity in detecting NFATc1 in nuclear extracts from Jurkat cells that are either unstimulated or stimulated for 6.5 hours with 1 μg/mL CD3.

Attributs du produit: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Ingrédients: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

These extracts are diluted down to 0.625 μg/well and assayed using the TransAM NFATc1 Kit. The ratio of the signals from the treated over the untreated cells must be above 3. Lot No. 32313013 was developed for 12 minutes. It gave a ratio of 4.8 (Figure 1). The basal level of NFATc1 expression, and this ratio, may vary depending on the cell type and the activation used. TransAM NFATc1 Kits are also tested for specificity in detecting NFATc1 activation. TransAM NFATc1 assays are performed in the presence of an excess of oligonucleotide containing a wild-type or mutated NFAT consensus binding site (Figure 2). At 20X excess, the wild-type oligonucleotide prevents NFATc1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has little or no effect on NFATc1 binding.

Durée du test: 5 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C/-80 °C

Stockage commentaire: Store the cell extract at -80°C. Other kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, DTT, and Protease Inhibitor Cocktail that should be kept at -20°C. This product is guaranteed for 6 months from date of receipt.

Date de péremption: 6 months

Détail du antigène

Antigène: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Autre désignation: Nfatc1 (NFATC1 Produits)

Synonymes: nf-atc Kit ELISA, nfat2 Kit ELISA, nfatc Kit ELISA, si:ch211-198c22.2 Kit ELISA, NFATC1 Kit ELISA, 2210017P03Rik Kit ELISA, AI449492 Kit ELISA, AV076380 Kit ELISA, NF-ATc Kit ELISA, NFAT2 Kit ELISA, NFATc Kit ELISA, Nfatcb Kit ELISA, NF-ATC Kit ELISA, NF-ATc1 Kit ELISA, NFAT Kit ELISA, NFATmac Kit ELISA, nuclear factor of activated T-cells 1 Kit ELISA, nuclear factor of activated T-cells 1 L homeolog Kit ELISA, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 Kit ELISA, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1 Kit ELISA, NFATC1 Kit ELISA, nfatc1.L Kit ELISA, nfatc1 Kit ELISA, Nfatc1 Kit ELISA

Pathways: Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway