CALCB Kit ELISA (Calcitonin-Related Polypeptide beta)

Details for Product CALCB ELISA Kit No. ABIN2871096
Antigène
  • CRSP
  • CRSP-1
  • CRSP1
  • CALCB
  • CALC2
  • CGRP-II
  • CGRP2
  • Calc2
  • calcitonin-related polypeptide beta
  • calcitonin related polypeptide beta
  • calcitonin gene-related peptide 2
  • calcitonin-related polypeptide, beta
  • CALCB
  • LOC698209
  • Calcb
Reactivité
Humain
Alternatives
Kits with alternative reactivity to:
7
7
7
Méthode de détection
Fluorometric
Type de méthode
Competition ELISA
Application
ELISA
Options
Analytical Method Quantitative
Méthode de détection Fluorometric
Plasmids, Primers & others Plasmids, Primers & others CALCB products on genomics-online (e.g. as negative or positive controls)
Antigène
Autre désignation Calcitonin Gene Related Peptide II (CGRP II) (CALCB ELISA Kit Extrait)
Pathways Hormone Activity
Indications d'application Optimal working dilution should be determined by the investigator.
Plaque Pre-coated
Protocole The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
Restrictions For Research Use only
Stock 4 °C
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