Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Caspase 3 Kit ELISA

CASP3 Reactivité: Humain AA 183-277 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate
N° du produit ABIN3044699
  • Antigène Voir toutes Caspase 3 (CASP3) Kits ELISA
    Caspase 3 (CASP3)
    Épitope
    AA 29-175, AA 183-277
    Reactivité
    • 8
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2-2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Caspase 3
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: S29-D175&A183-H277
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Immunogen sequence: S29-D175&A183-H277
    Featured
    Discover our best selling CASP3 Kit ELISA
    Top Product
    Discover our top product CASP3 Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.

    Plaque
    Pre-coated
    Protocole
    human Caspase 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Caspase 3 has been precoated onto 96-well plates. Standards(Expression system for standard: E. coli, Immunogen sequence: S29-D175 & A183-H277) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Caspase 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Caspase 3 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human Caspase 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, tissue homogenates or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Caspase 3 standard solution and each sample be measured in duplicate.

    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    4 °C,-20 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes Caspase 3 (CASP3) Kits ELISA
    Caspase 3 (CASP3)
    Autre désignation
    CASP3 (CASP3 Produits)
    Synonymes
    CPP32 Kit ELISA, CPP32B Kit ELISA, SCA-1 Kit ELISA, A830040C14Rik Kit ELISA, AC-3 Kit ELISA, Apopain Kit ELISA, CC3 Kit ELISA, Caspase-3 Kit ELISA, Lice Kit ELISA, Yama Kit ELISA, mldy Kit ELISA, xcpp32 Kit ELISA, casp3 Kit ELISA, zgc:100890 Kit ELISA, CASP-3 Kit ELISA, caspase-3 Kit ELISA, caspase 3 Kit ELISA, caspase 3 S homeolog Kit ELISA, caspase 3, apoptosis-related cysteine peptidase a Kit ELISA, caspase 3, apoptosis-related cysteine peptidase Kit ELISA, CASP3 Kit ELISA, Casp3 Kit ELISA, casp3.S Kit ELISA, casp3a Kit ELISA
    Sujet

    Protein Function: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. .

    Background: Caspase-3 is a caspase protein that is encoded by the CASP3 gene. This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

    Synonyms: Caspase-3,CASP-3,3.4.22.56,Apopain,Cysteine protease CPP32,CPP-32,Protein Yama,SREBP cleavage activity 1,SCA-1,Caspase-3 subunit p17,Caspase-3 subunit p12,CASP3,CPP32,

    Full Gene Name: Caspase-3

    Cellular Localisation: Cytoplasm.
    ID gène
    836
    UniProt
    P42574
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
Vous êtes ici:
Support technique