FLT1 Kit ELISA

N° du produit ABIN411372

Détail du produit FLT1 Kit ELISA

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Épitope: AA 27-687

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156-10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human sVEGFR1/sFLT1

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21
Immunogen sequence: S27-H687

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <30pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: S27-H687

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Tissue Specificity: Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC). .

Plaque: Pre-coated

Protocole: human sVEGFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for sVEGFR1 has been precoated onto 96-well plates. Standards(sf21, S27-H687) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for sVEGFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human sVEGFR1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human sVEGFR1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human sVEGFR1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 367, Standard deviation: 15.41, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 2835, Standard deviation: 130.4, CV(%): 4.6
• Sample 3: n=16, Mean(pg/ml): 5213, Standard deviation: 255.4, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 530, Standard deviation: 27.56, CV(%): 5.2
• Sample 2: n=24, Mean(pg/ml): 3015, Standard deviation: 180.9, CV(%): 6
• Sample 3: n=24, Mean(pg/ml): 6142, Standard deviation: 399.2, CV(%): 6.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour FLT1 Kit ELISA (ABIN411372)

Bozduman, Ersoy Evans, Karahan, Hayran, Akbiyik, Lay: "Genetic Risk Factors for Psoriasis in Turkish Population: -1540 C/A, -1512 Ins18, and +405 C/G Polymorphisms within the Vascular Endothelial Growth Factor Gene." dans: Annals of dermatology, Vol. 28, Issue 1, pp. 30-9, (2016) (PubMed).

Bakir, Bakir, Sari, Celik, Gozel, Engin: "Evaluation of the relationship between serum levels of VEGF and sVEGFR1 with mortality and prognosis in patients with Crimean-Congo hemorrhagic fever." dans: Journal of medical virology, Vol. 85, Issue 10, pp. 1794-801, (2013) (PubMed).

Liu, Li, Su, Huang, Luo: "Soluble Fms-like tyrosine kinase-1 expression inhibits the growth of multiple myeloma in nude mice." dans: Acta biochimica et biophysica Sinica, Vol. 39, Issue 7, pp. 499-506, (2007) (PubMed).

Détail du antigène

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Autre désignation: FLT1 (FLT1 Produits)

Synonymes: FLT Kit ELISA, FLT-1 Kit ELISA, VEGFR-1 Kit ELISA, VEGFR1 Kit ELISA, FLT1 Kit ELISA, flt1b Kit ELISA, sFlt1 Kit ELISA, AI323757 Kit ELISA, Flt-1 Kit ELISA, fms related tyrosine kinase 1 Kit ELISA, FMS-related tyrosine kinase 1 Kit ELISA, vascular endothelial growth factor receptor 1 Kit ELISA, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor) Kit ELISA, FMS-like tyrosine kinase 1 Kit ELISA, vascular endothelial growth factor receptor-1 Kit ELISA, FLT1 Kit ELISA, Flt1 Kit ELISA, CpipJ_CPIJ012087 Kit ELISA, flt1 Kit ELISA, FLT-1 Kit ELISA

Sujet:

Protein Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity, may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates SRC and YES1, and may also phosphorylate CBL. Isoform 1 phosphorylates PLCG. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1. Isoform 7 has a truncated kinase domain, it increases phosphorylation of SRC at 'Tyr-418' by unknown means and promotes tumor cell invasion. .

Background: sVEGFR1, also known as sFMS-related tyrosine kinase 1(sFLT1). Oncogene sFLT belongs to the src gene family and is related to oncogene ROS . Like other members of this family, it shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. sFLT is mapped to 13q12. sVEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. The standard product used in this kit is recombinant human sVEGFR1, consisting of 905 amino acids with the molecular mass of 100KDa.

Synonyms: Vascular endothelial growth factor receptor 1,VEGFR-1,2.7.10.1,Fms-like tyrosine kinase 1,FLT-1,Tyrosine-protein kinase FRT,Tyrosine-protein kinase receptor FLT,FLT,Vascular permeability factor receptor,FLT1,FLT, FRT, VEGFR1,

Full Gene Name: Vascular endothelial growth factor receptor 1

Cellular Localisation: Isoform 1: Cell membrane, Single-pass type I membrane protein. Endosome. Autophosphorylation promotes ubiquitination and endocytosis.

ID gène: 2321

UniProt: P17948

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals