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CXCL1 Kit ELISA

CXCL1 Reactivité: Rat AA 25-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
N° du produit ABIN411394
  • Antigène Voir toutes CXCL1 Kits ELISA
    CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
    Épitope
    AA 25-96
    Reactivité
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CXCL1
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: E.coli
    Immunogen sequence: A25-K96
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <1pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: E.coli
    Immunogen sequence: A25-K96
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Tissue Specificity: At least expressed in the lung and trachea.

    Plaque
    Pre-coated
    Protocole
    rat CXCL1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL1 has been precoated onto 96-well plates. Standards(E. coli, A25-K96) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CXCL1 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL rat CXCL1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma(EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat CXCL1 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 75.4, Standard deviation: 4.1, CV(%): 5.4
    • Sample 2: n=16, Mean(pg/ml): 221, Standard deviation: 13.7, CV(%): 6.2
    • Sample 3: n=16, Mean(pg/ml): 546, Standard deviation: 28.4, CV(%): 5.2,
    • Sample 1: n=24, Mean(pg/ml): 98.4, Standard deviation: 6, CV(%): 6.1
    • Sample 2: n=24, Mean(pg/ml): 285, Standard deviation: 20.52, CV(%): 7.2
    • Sample 3: n=24, Mean(pg/ml): 534, Standard deviation: 31.5, CV(%): 5.9
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Fu, Wang, Cai, Zhao, Fu: "Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa β-related pathway in rats." dans: Drug design, development and therapy, Vol. 12, pp. 1609-1619, (2018) (PubMed).

    Zhang, Wang, Liu, Zhang, Chen: "Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α." dans: Gastroenterology research and practice, Vol. 2016, pp. 6421351, (2016) (PubMed).

  • Antigène Voir toutes CXCL1 Kits ELISA
    CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
    Autre désignation
    CXCL1 (CXCL1 Produits)
    Synonymes
    FSP Kit ELISA, GRO1 Kit ELISA, GROa Kit ELISA, MGSA Kit ELISA, MGSA-a Kit ELISA, NAP-3 Kit ELISA, SCYB1 Kit ELISA, CINC-1 Kit ELISA, Gro1 Kit ELISA, CXCL1 Kit ELISA, Sdf1 Kit ELISA, Fsp Kit ELISA, KC Kit ELISA, Mgsa Kit ELISA, N51 Kit ELISA, Scyb1 Kit ELISA, gro Kit ELISA, GRO Kit ELISA, Gro Kit ELISA, C-X-C motif chemokine ligand 1 Kit ELISA, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) Kit ELISA, C-X-C motif chemokine ligand 12 Kit ELISA, chemokine (C-X-C motif) ligand 1 Kit ELISA, CXCL1 Kit ELISA, Cxcl1 Kit ELISA, Cxcl12 Kit ELISA, GRO1 Kit ELISA
    Sujet

    Protein Function: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.

    Background: Chemokine(C-X-C motif) ligand 1(CXCL1) is a small cytokine belonging to the CXC chemokine family that was previously called GRO1 oncogene, GROalpha, KC, Neutrophil-activating protein 3(NAP-3) and melanoma growth stimulating activity, alpha(MSGA-alpha). In humans, this protein is encoded by the CXCL1 gene. The gene for CXCL1 is located on human chromosome 4 amongst genes for other CXC chemokines. The mature form of CXCL1 is maximally 73 amino acids long. CXCL1 is secreted by human melanoma cells, has mitogenic properties and is implicated in melanoma pathogenesis. CXCL1 is expressed by macrophages, neutrophils and epithelial cells, and has neutrophil chemoattractant activity. This chemokine elicits its effects by signaling through the chemokine receptor CXCR2.CXCL1 decreased the severity of multiple sclerosis and may offer a neuro-protective function.

    Synonyms: Growth-regulated alpha protein,C-X-C motif chemokine 1,Cytokine-induced neutrophil chemoattractant 1,CINC-1,Platelet-derived growth factor-inducible protein KC,Cxcl1,Cinc1, Gro, Scyb1,

    Full Gene Name: Growth-regulated alpha protein

    Cellular Localisation: Secreted.
    ID gène
    81503
    UniProt
    P14095
    Pathways
    Autophagy
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