Attributs du produit
ELISA-VIDITEST anti HSV1+2 IgG (CSF) assay is intended for in vitro diagnosis of HSV type 1 or 2 associated diseases, i.e. herpes labialis, herpes genitalis, herpesvirus gingivostomatitis, keratoconjunctivitis and herpesvirus-induced neurological complications (encephalitis, meningitis, inflammatory mono- and polyneuropathies). The diagnostic kit can be also utilized for differential diagnosis of neuroinfections, infections of eye and skin and exanthematous diseases. The polystyrene strips are coated with a mixture of antigens which bear immunodominant epitopes of HSV1 and HSV2. Anti-HSV1+2 antibodies from serum samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away, those that formed complexes with the antigens are later on recognised by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react and the mild change in colour, if present, may be attributed to the reaction background. Detection of intrathecal production of antibodies (detection of specific IgG antibodies in cerebrospinal fluid produced intrathecally) is necessary for the assessment of the antibody response within the central nervous system. It requires parallel measurements of specific IgG antibodies in serum and in cerebrospinal fluid sample taken from the patient at the same time and the determination of total albumin and total IgG in both samples. The calculation of the specific antibody production is according to Reiber equation.