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Substance P Kit ELISA

Reactivité: Humain, Souris, Rat Colorimetric Competition ELISA 0.1-1.000 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4881375
  • Antigène Voir toutes Substance P Kits ELISA
    Substance P
    Reactivité
    • 7
    • 6
    • 6
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Competition ELISA
    Gamme de detection
    0.1-1.000 ng/mL
    Seuil minimal de détection
    0.1 ng/mL
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Substance P EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This EIA kit is designed to detect human, mouse, and rat Substance P.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Standard Peptide
    • Assay Diluent(s)
    • Biotinylated Peptide
    • HRP-Streptavidin
    • TMB One-Step Substrate
    • Stop Solution
    • Assay Diagram
    • Positive Control Sample
    • Capture Antibody
    • User Manual
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Orbital shaker
    • Aluminum foil
    • Saran Wrap
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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  • Indications d'application
    Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 8x / Rat: 8X
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed.
    2. Add 100 μL detection antibody to each well.
    3. Incubate 1.5 h at RT or O/N at 4 °C.
    4. Add 100 μL standard or sample to each well.
    5. Incubate 2.5 h at RT.
    6. Add 100 μL prepared streptavidin solution.
    7. Incubate 45 min at RT.
    8. Add 100 μL TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL Stop Solution to each well.
    11. Read plate at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
    Date de péremption
    6 months
  • Antigène Voir toutes Substance P Kits ELISA
    Substance P
    Abstract
    Substance P Produits
    Synonymes
    Hs.2563 Kit ELISA, NK2 Kit ELISA, NKNA Kit ELISA, NPK Kit ELISA, TAC2 Kit ELISA, PPTA3 Kit ELISA, Ppt5fl Kit ELISA, RATPPTA3 Kit ELISA, TAC Kit ELISA, 4930528L02Rik Kit ELISA, NK-1 Kit ELISA, NK1 Kit ELISA, Nkna Kit ELISA, PPT-A Kit ELISA, PPTA Kit ELISA, SP Kit ELISA, PPT Kit ELISA, beta-PPT-A Kit ELISA, NKA Kit ELISA, tachykinin precursor 1 Kit ELISA, tachykinin, precursor 1 Kit ELISA, tachykinin 1 Kit ELISA, TAC1 Kit ELISA, Tac1 Kit ELISA
    ID gène
    21333
    UniProt
    P41539
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