NUCB2 Kit ELISA
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- Antigène Voir toutes NUCB2 Kits ELISA
- NUCB2 (Nucleobindin 2 (NUCB2))
- Reactivité
- Rat, Humain, Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 0.1-1.000 ng/mL
- Seuil minimal de détection
- 0.1 ng/mL
- Application
- ELISA
- Fonction
- Human/Mouse/Rat Nucleobindin-2 EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
- Type d'échantillon
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
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This kit detects 420aa Nucleobindin-2 protein.
Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Angiotensin II, NPY and APC. - Sensibilité
- 147 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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- Indications d'application
- Recommended Dilution for serum and plasma samplesHuman: 4X / Mouse: 4X / Rat: 2X
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
- Date de péremption
- 6 months
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- Antigène Voir toutes NUCB2 Kits ELISA
- NUCB2 (Nucleobindin 2 (NUCB2))
- Autre désignation
- Nucleobindin-2 (NUCB2 Produits)
- Synonymes
- nefa Kit ELISA, MGC97715 Kit ELISA, NUCB2 Kit ELISA, nucleobindin-2 Kit ELISA, NEFA Kit ELISA, AI607786 Kit ELISA, Calnuc Kit ELISA, Nefa Kit ELISA, Nesfatin-1 Kit ELISA, p54 Kit ELISA, nucleobindin 2 Kit ELISA, nucleobindin 2 L homeolog Kit ELISA, nucb2 Kit ELISA, NUCB2 Kit ELISA, Nucb2 Kit ELISA, nucb2.L Kit ELISA
- UniProt
- Q9JI85
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