VEGFA Kit ELISA
-
- Antigène Voir toutes VEGFA Kits ELISA
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
-
Reactivité
- Boeuf (Vache)
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.123-30 ng/mL
- Seuil minimal de détection
- 0.123 ng/mL
- Application
- ELISA
- Fonction
- Bovine VEGF-A ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes Bovine VEGF-A.
- Sensibilité
- 0.12 ng/mL
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling VEGFA Kit ELISA
- Top Product
- Discover our top product VEGFA Kit ELISA
-
-
- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
-
- Antigène Voir toutes VEGFA Kits ELISA
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- Autre désignation
- VEGF-A (VEGFA Produits)
- Synonymes
- MVCD1 Kit ELISA, VEGF Kit ELISA, VPF Kit ELISA, Vegf Kit ELISA, Vegf120 Kit ELISA, Vegf164 Kit ELISA, Vegf188 Kit ELISA, Vpf Kit ELISA, vegf Kit ELISA, vegfa Kit ELISA, wu:fj82c06 Kit ELISA, VEGF-A Kit ELISA, VEGF164 Kit ELISA, eVEGF120 Kit ELISA, eVEGF164 Kit ELISA, vegf-a Kit ELISA, vpf Kit ELISA, vefg Kit ELISA, si:dkey-14d8.3 Kit ELISA, wu:fd42e02 Kit ELISA, vascular endothelial growth factor A Kit ELISA, vascular endothelial growth factor Aa Kit ELISA, vascular endothelial growth factor A L homeolog Kit ELISA, vascular endothelial growth factor Ab Kit ELISA, VEGFA Kit ELISA, Vegfa Kit ELISA, vegfaa Kit ELISA, vegfa.L Kit ELISA, vegfa Kit ELISA, vegfab Kit ELISA
- ID gène
- 281572
- UniProt
- P15691
- Pathways
- Signalisation RTK, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
-