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Caspase 8 Kit ELISA

CASP8 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882103
  • Antigène Voir toutes Caspase 8 (CASP8) Kits ELISA
    Caspase 8 (CASP8)
    Reactivité
    • 7
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Caspase-8 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human FHL1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Caspase 8 (CASP8) Kits ELISA
    Caspase 8 (CASP8)
    Autre désignation
    Caspase-8 (CASP8 Produits)
    Synonymes
    casp8-A Kit ELISA, xCaspase-8 Kit ELISA, casp8 Kit ELISA, CASP8 Kit ELISA, CASP-8 Kit ELISA, FLICE Kit ELISA, MACH Kit ELISA, Mch5 Kit ELISA, ALPS2B Kit ELISA, CAP4 Kit ELISA, Casp-8 Kit ELISA, MCH5 Kit ELISA, caspase-8 Kit ELISA, zgc:92075 Kit ELISA, caspase 8 L homeolog Kit ELISA, death related ced-3/Nedd2-like protein Kit ELISA, caspase 8 Kit ELISA, caspase-8 Kit ELISA, caspase-8-like Kit ELISA, caspase 8, apoptosis-related cysteine peptidase Kit ELISA, casp8.L Kit ELISA, LOC661095 Kit ELISA, CASP8 Kit ELISA, LOC5563818 Kit ELISA, casp8 Kit ELISA, LOC100222284 Kit ELISA, Casp8 Kit ELISA
    ID gène
    841
    UniProt
    Q14790
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
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