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CLEC10A Kit ELISA

CLEC10A Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882372
  • Antigène Voir toutes CLEC10A Kits ELISA
    CLEC10A (C-Type Lectin Domain Family 10, Member A (CLEC10A))
    Reactivité
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human CLEC10A (CD301) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human CLEC10A. Other species not yet determined.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product CLEC10A Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CLEC10A Kits ELISA
    CLEC10A (C-Type Lectin Domain Family 10, Member A (CLEC10A))
    Autre désignation
    CLEC10A (CLEC10A Produits)
    Synonymes
    CLEC10A Kit ELISA, CD301 Kit ELISA, CLECSF13 Kit ELISA, CLECSF14 Kit ELISA, HML Kit ELISA, HML2 Kit ELISA, MGL Kit ELISA, CD301a Kit ELISA, M-ASGP-BP-1 Kit ELISA, Mgl Kit ELISA, Mgl1 Kit ELISA, Mgll Kit ELISA, C-type lectin domain family 10 member A Kit ELISA, C-type lectin domain containing 10A Kit ELISA, C-type lectin domain family 10, member A Kit ELISA, LOC607405 Kit ELISA, CLEC10A Kit ELISA, Clec10a Kit ELISA
    ID gène
    10462
    UniProt
    Q8IUN9
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