HSPD1 Kit ELISA

N° du produit ABIN4883232

Détail du produit HSPD1 Kit ELISA

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human HSP60 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human HSP60.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Autre désignation: HSP60 (HSPD1 Produits)

Synonymes: CPN60 Kit ELISA, GROEL Kit ELISA, HLD4 Kit ELISA, HSP-60 Kit ELISA, HSP60 Kit ELISA, HSP65 Kit ELISA, HuCHA60 Kit ELISA, SPG13 Kit ELISA, chaperonin Kit ELISA, cpn60 Kit ELISA, groel Kit ELISA, hld4 Kit ELISA, hsp60 Kit ELISA, hsp65 Kit ELISA, spg13 Kit ELISA, 60kDa Kit ELISA, Hsp60 Kit ELISA, 12 Kit ELISA, BP5 Kit ELISA, CG12101 Kit ELISA, Cpn60 Kit ELISA, Dmel\\CG12101 Kit ELISA, Dmhsp60 Kit ELISA, G62 Kit ELISA, HSP60A Kit ELISA, Hsp60A Kit ELISA, IEF16 Kit ELISA, Mmp-P1 Kit ELISA, SSP 7506 Kit ELISA, d-hsp60 Kit ELISA, hsp60A Kit ELISA, l(1)10Ac Kit ELISA, l(1)BP5 Kit ELISA, l(1)G8 Kit ELISA, l(1)HM21 Kit ELISA, l(1)L12 Kit ELISA, l(1)dp025 Kit ELISA, cb863 Kit ELISA, fa04a05 Kit ELISA, fb22d10 Kit ELISA, fi27b05 Kit ELISA, id:ibd2197 Kit ELISA, sb:cb144 Kit ELISA, wu:fa04a05 Kit ELISA, wu:fb22d10 Kit ELISA, wu:fi04a12 Kit ELISA, wu:fi27b05 Kit ELISA, MIF4 Kit ELISA, MNA2 Kit ELISA, mopA Kit ELISA, groL Kit ELISA, crpA Kit ELISA, Hspd1-30p Kit ELISA, heat shock protein family D (Hsp60) member 1 Kit ELISA, heat shock protein family D (Hsp60) member 1 S homeolog Kit ELISA, 60 kDa heat shock protein, mitochondrial Kit ELISA, heat shock protein 1 (chaperonin) Kit ELISA, Heat shock protein 60A Kit ELISA, heat shock 60 protein 1 Kit ELISA, chaperone ATPase HSP60 Kit ELISA, molecular chaperone GroEL Kit ELISA, thermosome subunit Kit ELISA, chaperonin GroEL Kit ELISA, mitochondrial chaperonin Kit ELISA, heat shock protein family D member 1 Kit ELISA, HSPD1 Kit ELISA, hspd1.S Kit ELISA, hspd1 Kit ELISA, LOC100414401 Kit ELISA, Hspd1 Kit ELISA, Hsp60A Kit ELISA, HSP60 Kit ELISA, groEL Kit ELISA, MMP_RS07785 Kit ELISA, groEl Kit ELISA, LOC100136430 Kit ELISA

ID gène: 3329

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity