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KPNA2 Kit ELISA

KPNA2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883449
  • Antigène Voir toutes KPNA2 Kits ELISA
    KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))
    Reactivité
    • 4
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Importin alpha 2/KPNA2/SRP1-alpha ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes KPNA2 Kits ELISA
    KPNA2 (Karyopherin alpha 2 (RAG Cohort 1, Importin alpha 1) (KPNA2))
    Autre désignation
    Importin alpha 2 (KPNA2 Produits)
    Synonymes
    IPOA1 Kit ELISA, QIP2 Kit ELISA, RCH1 Kit ELISA, SRP1alpha Kit ELISA, ima2 Kit ELISA, importin Kit ELISA, 2410044B12Rik Kit ELISA, PTAC58 Kit ELISA, Rch1 Kit ELISA, ipoa1 Kit ELISA, kpna2 Kit ELISA, qip2 Kit ELISA, rch1 Kit ELISA, srp1alpha Kit ELISA, hm:zeh0389 Kit ELISA, hm:zeh0389r Kit ELISA, zgc:113902 Kit ELISA, zgc:86945 Kit ELISA, karyopherin subunit alpha 2 Kit ELISA, importin subunit alpha-2 Kit ELISA, Importin subunit alpha-2 Kit ELISA, karyopherin (importin) alpha 2 Kit ELISA, karyopherin alpha-2 subunit like L homeolog Kit ELISA, karyopherin alpha 2 (RAG cohort 1, importin alpha 1) Kit ELISA, KPNA2 Kit ELISA, EDI_246290 Kit ELISA, EDI_335040 Kit ELISA, ima2 Kit ELISA, Kpna2 Kit ELISA, kpna2.L Kit ELISA, kpna2 Kit ELISA
    ID gène
    3838
    UniProt
    P52292
    Pathways
    M Phase, Protein targeting to Nucleus
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