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MMP7 Kit ELISA

MMP7 Reactivité: Humain Colorimetric Sandwich ELISA 0.15-100 ng/mL Cell Culture Supernatant
N° du produit ABIN4883827
  • Antigène Voir toutes MMP7 Kits ELISA
    MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
    Reactivité
    • 7
    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.15-100 ng/mL
    Seuil minimal de détection
    0.15 ng/mL
    Application
    ELISA
    Fonction
    Human MMP-7 ELISA Kit for Cell Culture Supernatants samples.
    Type d'échantillon
    Cell Culture Supernatant
    Analytical Method
    Quantitative
    Specificité
    This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, 9, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensibilité
    150 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling MMP7 Kit ELISA
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    Discover our top product MMP7 Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples10 - 100 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Garratt, Sutanto, Ling, Looi, Iosifidis, Martinovich, Shaw, Kicic-Starcevich, Knight, Ranganathan, Stick, Kicic: "Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis." dans: The European respiratory journal, Vol. 46, Issue 2, pp. 384-94, (2015) (PubMed).

    Erbel, Tyka, Helmes, Akhavanpoor, Rupp, Domschke, Linden, Wolf, Doesch, Lasitschka, Katus, Gleissner: "CXCL4-induced plaque macrophages can be specifically identified by co-expression of MMP7+S100A8+ in vitro and in vivo." dans: Innate immunity, Vol. 21, Issue 3, pp. 255-65, (2015) (PubMed).

    Manjari, Jyothy, Kumar, Prabhakar, Nallari, Venkateshwari: "Association of matrix metalloproteinase-7 (-181A/G) promoter polymorphism in chronic pancreatitis." dans: The Indian journal of medical research, Vol. 140, Issue 5, pp. 609-15, (2015) (PubMed).

    Zhang, Shively: "Acceleration of bone repair in NOD/SCID mice by human monoosteophils, novel LL-37-activated monocytes." dans: PLoS ONE, Vol. 8, Issue 7, pp. e67649, (2013) (PubMed).

    Hickman, Sargent: "The application of the use of radioactive phosphorus (32P) in larvae: a new approach for identification of adult insect sources." dans: Investigative radiology, Vol. 1, Issue 2, pp. 104-5 (PubMed).

  • Antigène Voir toutes MMP7 Kits ELISA
    MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))
    Autre désignation
    MMP-7 (MMP7 Produits)
    Synonymes
    MAT Kit ELISA, MPMM Kit ELISA, MMP-7 Kit ELISA, MPSL1 Kit ELISA, PUMP-1 Kit ELISA, matrilysin Kit ELISA, LOC727698 Kit ELISA, MMP7 Kit ELISA, Matrilysin Kit ELISA, matrix metallopeptidase 7 Kit ELISA, MMP7 Kit ELISA, Mmp7 Kit ELISA
    Sujet
    Gene Names: MMP7 MPSL1 PUMP1
    Protein names: Matrilysin (EC 3.4.24.23) (Matrin) (Matrix metalloproteinase-7) (MMP-7) (Pump-1 protease) (Uterine metalloproteinase)
    ID gène
    4316
    UniProt
    P09237
    Pathways
    Production of Molecular Mediator of Immune Response
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