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PCNA Kit ELISA

PCNA Reactivité: Humain Colorimetric Sandwich ELISA 2.5-600 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884109
  • Antigène Voir toutes PCNA Kits ELISA
    PCNA (Proliferating Cell Nuclear Antigen (PCNA))
    Reactivité
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    2.5-600 ng/mL
    Seuil minimal de détection
    2.5 ng/mL
    Application
    ELISA
    Fonction
    Human PCNA ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Human PCNA
    Sensibilité
    2.46 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes PCNA Kits ELISA
    PCNA (Proliferating Cell Nuclear Antigen (PCNA))
    Autre désignation
    PCNA (PCNA Produits)
    Synonymes
    PCNAR Kit ELISA, Pcna/cyclin Kit ELISA, pcna Kit ELISA, AO090003000846 Kit ELISA, 53/13 Kit ELISA, CG9193 Kit ELISA, DmPCNA Kit ELISA, Dmel\\CG9193 Kit ELISA, MUS209 Kit ELISA, Mus209 Kit ELISA, PCNA Kit ELISA, PCNA/mus209 Kit ELISA, Pcna Kit ELISA, dPCNA Kit ELISA, l(2)02448 Kit ELISA, l(2)56F Kit ELISA, l(2)56Fa Kit ELISA, l(2)s1534 Kit ELISA, cb16 Kit ELISA, fb36g03 Kit ELISA, wu:fa28e03 Kit ELISA, wu:fb36g03 Kit ELISA, pcna-A Kit ELISA, proliferating cell nuclear antigen Kit ELISA, DNA polymerase III sliding clamp Kit ELISA, Proliferating cell nuclear antigen Kit ELISA, Proliferating cell nuclear antigen-like Kit ELISA, proliferating cell nuclear antigen L homeolog Kit ELISA, proliferating cell nuclear antigen 1 Kit ELISA, PCNA Kit ELISA, Pcna Kit ELISA, pcna Kit ELISA, MMP_RS08810 Kit ELISA, ANI_1_512014 Kit ELISA, AOR_1_1500154 Kit ELISA, MGC53867 Kit ELISA, pcna.L Kit ELISA, pcna1 Kit ELISA, LOC106420145 Kit ELISA, pcn1 Kit ELISA
    ID gène
    5111
    UniProt
    P12004
    Pathways
    Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
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