PLA2G4A Kit ELISA

N° du produit ABIN4884173

Détail du produit PLA2G4A Kit ELISA

Antigène: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human PLA2G4A ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: PLA2G4A (Phospholipase A2, Group IVA (Cytosolic, Calcium-Dependent) (PLA2G4A))

Autre désignation: PLA2G4A (PLA2G4A Produits)

Synonymes: PLA2G4 Kit ELISA, cPLA2-alpha Kit ELISA, Pla2c Kit ELISA, Pla2g4 Kit ELISA, cPLA2 Kit ELISA, pla2g4 Kit ELISA, CPLA2 Kit ELISA, PLA2G4A Kit ELISA, PLA2 Kit ELISA, PLA2G1B Kit ELISA, cPLA2alpha Kit ELISA, pla2g4a Kit ELISA, si:dkey-97o5.1 Kit ELISA, phospholipase A2 group IVA Kit ELISA, phospholipase A2 group IVA L homeolog Kit ELISA, phospholipase A2, group IVA (cytosolic, calcium-dependent) Kit ELISA, phospholipase A2, group IVAb (cytosolic, calcium-dependent) Kit ELISA, PLA2G4A Kit ELISA, Pla2g4a Kit ELISA, pla2g4a.L Kit ELISA, pla2g4ab Kit ELISA

ID gène: 5321

UniProt: P47712

Pathways: Inositol Metabolic Process, G-protein mediated Events, VEGF Signaling