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PSMA1 Kit ELISA

PSMA1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4884280
  • Antigène Voir toutes PSMA1 Kits ELISA
    PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human PSMA1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes PSMA1 Kits ELISA
    PSMA1 (Proteasome Subunit alpha Type 1 (PSMA1))
    Autre désignation
    PSMA1 (PSMA1 Produits)
    Synonymes
    PSMA1 Kit ELISA, pros30 Kit ELISA, DDBDRAFT_0204226 Kit ELISA, DDBDRAFT_0214956 Kit ELISA, DDB_0204226 Kit ELISA, DDB_0214956 Kit ELISA, C2 Kit ELISA, HC2 Kit ELISA, Pros-30 Kit ELISA, alpha-type Kit ELISA, NU Kit ELISA, PROS30 Kit ELISA, CC2 Kit ELISA, zgc:92726 Kit ELISA, 143314_at Kit ELISA, Alpha-6 Kit ELISA, CG4904 Kit ELISA, Dmel\CG4904 Kit ELISA, PROS-35 Kit ELISA, PROS-Dm35 Kit ELISA, Pros-35 Kit ELISA, Pros-Dm35 Kit ELISA, Prosalpha6 Kit ELISA, alpha6 Kit ELISA, alpha_dm Kit ELISA, anon-SAGE:Wang-116 Kit ELISA, pros 35 Kit ELISA, pros35 Kit ELISA, proteasome subunit alpha 1 Kit ELISA, proteasome subunit alpha type 1 Kit ELISA, 20S proteasome subunit C2 Kit ELISA, proteasome (prosome, macropain) subunit, alpha type 1 Kit ELISA, proteasome subunit alpha 1 L homeolog Kit ELISA, Proteasome alpha6 subunit Kit ELISA, PSMA1 Kit ELISA, psma1 Kit ELISA, CNB05540 Kit ELISA, psmA1 Kit ELISA, Psma1 Kit ELISA, psma1.L Kit ELISA, Prosalpha6 Kit ELISA
    ID gène
    5682
    UniProt
    P25786
    Pathways
    Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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