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RAGE Kit ELISA

AGER Reactivité: Humain Colorimetric Sandwich ELISA 3-1500 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884312
  • Antigène Voir toutes RAGE (AGER) Kits ELISA
    RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
    Reactivité
    • 7
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    • 3
    • 2
    • 1
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    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    3-1500 pg/mL
    Seuil minimal de détection
    3 pg/mL
    Application
    ELISA
    Fonction
    Human RAGE ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
    Sensibilité
    3 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling AGER Kit ELISA
    Top Product
    Discover our top product AGER Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples2 - 20 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Nirala, Perumal, Gohil: "Glycated serum albumin stimulates expression of endothelial cell specific molecule-1 in human umbilical vein endothelial cells: Implication in diabetes mediated endothelial dysfunction." dans: Diabetes & vascular disease research, Vol. 12, Issue 4, pp. 290-7, (2015) (PubMed).

    Nirala, Gohil: "Effect of garlic component s-allyl cysteine sulfoxide on glycated human serum albumin induced activation of endothelial cells: an in vitro study." dans: European review for medical and pharmacological sciences, Vol. 19, Issue 11, pp. 2125-31, (2015) (PubMed).

    Bala, Gohil: "Interaction of glycated protein and DFO mimicked hypoxia in cellular responses of HUVECs." dans: Molecular bioSystems, Vol. 8, Issue 10, pp. 2657-63, (2012) (PubMed).

    Bala, Gomes, Gohil: "Interaction of glycated human serum albumin with endothelial cells in a hemodynamic environment: structural and functional correlates." dans: Molecular bioSystems, Vol. 7, Issue 11, pp. 3036-41, (2012) (PubMed).

    Chima, LaMontagne, Piraino, Hake, Denenberg, Zingarelli: "C-peptide, a novel inhibitor of lung inflammation following hemorrhagic shock." dans: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 5, pp. L730-9, (2011) (PubMed).

  • Antigène Voir toutes RAGE (AGER) Kits ELISA
    RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))
    Autre désignation
    RAGE (AGER Produits)
    Synonymes
    RAGE Kit ELISA, AGER Kit ELISA, advanced glycosylation end-product specific receptor Kit ELISA, advanced glycosylation end product-specific receptor Kit ELISA, MAPK/MAK/MRK overlapping kinase Kit ELISA, AGER Kit ELISA, Ager Kit ELISA, LOC719012 Kit ELISA
    Sujet
    Gene Names: MOK RAGE RAGE1
    Protein names: MAPK/MAK/MRK overlapping kinase (EC 2.7.11.22) (MOK protein kinase) (Renal tumor antigen 1) (RAGE-1)
    ID gène
    5891
    UniProt
    Q9UQ07
    Pathways
    Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins
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