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RBP4 Kit ELISA

RBP4 Reactivité: Humain Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884322
  • Antigène Voir toutes RBP4 Kits ELISA
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    Reactivité
    • 8
    • 8
    • 8
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.1-30 ng/mL
    Seuil minimal de détection
    0.1 ng/mL
    Application
    ELISA
    Fonction
    Human RBP4 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects human RBP4. This antibody pair does not cross-react with mouse RBP4.
    Sensibilité
    0.1 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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    Discover our best selling RBP4 Kit ELISA
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    Discover our top product RBP4 Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples10,000 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Ghareeb, Khalil, Hafez, Bajorath, Ahmed, Sarhan, Elwakeel, El-Demellawy: "Berberine Reduces Neurotoxicity Related to Nonalcoholic Steatohepatitis in Rats." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 361847, (2015) (PubMed).

  • Antigène Voir toutes RBP4 Kits ELISA
    RBP4 (Retinol Binding Protein 4, Plasma (RBP4))
    Autre désignation
    RBP-4 (RBP4 Produits)
    Synonymes
    RDCCAS Kit ELISA, RBP Kit ELISA, RNBP Kit ELISA, rbp4 Kit ELISA, MGC54038 Kit ELISA, srbp Kit ELISA, PRBP Kit ELISA, RBPA Kit ELISA, Rbp-4 Kit ELISA, fb23c12 Kit ELISA, rbp Kit ELISA, wu:fb23c12 Kit ELISA, wu:fb58d04 Kit ELISA, wu:fb72b04 Kit ELISA, zgc:86911 Kit ELISA, retinol binding protein 4 Kit ELISA, renin binding protein Kit ELISA, retinol binding protein 4 S homeolog Kit ELISA, retinol binding protein 4 A, plasma Kit ELISA, retinol binding protein 4, plasma Kit ELISA, retinol binding protein 4 L homeolog Kit ELISA, RBP4 Kit ELISA, RENBP Kit ELISA, rbp4.S Kit ELISA, rbp4 Kit ELISA, Rbp4 Kit ELISA, RBP4A Kit ELISA, rbp4.L Kit ELISA
    Sujet
    Gene Names: RBP4 PRO2222
    Protein names: Retinol-binding protein 4 (Plasma retinol-binding protein) (PRBP) (RBP) [Cleaved into: Plasma retinol-binding protein(1-182), Plasma retinol-binding protein(1-181), Plasma retinol-binding protein(1-179), Plasma retinol-binding protein(1-176)]
    ID gène
    5950
    UniProt
    P02753
    Pathways
    Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response
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