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SERPINA5 Kit ELISA
SERPINA5
Reactivité: Humain
Colorimetric
Sandwich ELISA
24.58-6.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4884478
Détail du produit SERPINA5 Kit ELISA
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Antigène
Voir toutes SERPINA5 Kits ELISA
SERPINA5
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 5 (SERPINA5))
Reactivité
Toutes les réactivités sur SERPINA5 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
24.58-6.000 pg/mL
Seuil minimal de détection
24.58 pg/mL
Application
ELISA
Fonction
Human Serpin A5/Protein C Inhibitor ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human Serpin A5.
Sensibilité
24 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2,000 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes SERPINA5 Kits ELISA
SERPINA5
(serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 5 (SERPINA5))
Autre désignation
SERPINA5 (SERPINA5 Produits )
Synonymes
4933415L04 Kit ELISA, PAI-3 Kit ELISA, Pci Kit ELISA, PAI3 Kit ELISA, PCI Kit ELISA, PCI-B Kit ELISA, PLANH3 Kit ELISA, PROCI Kit ELISA, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5 Kit ELISA, serpin family A member 5 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade A, member 5 Kit ELISA, plasma serine protease inhibitor Kit ELISA, SPIA5 Kit ELISA, SERPINA5 Kit ELISA, Serpina5 Kit ELISA, LOC480424 Kit ELISA
ID gène
5104
UniProt
P05154
Vus récemment
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