SERPIND1 Kit ELISA

N° du produit ABIN4884490

Détail du produit SERPIND1 Kit ELISA

Antigène: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.23-300 ng/mL

Seuil minimal de détection: 1.23 ng/mL

Application: ELISA

Fonction: Human SERPIND1 (Heparin Cofactor II) ELISA Kit for serum, plasma, and cell culture supernatant samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human Serpin D1. Other species not determined.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples20,000 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: SERPIND1 (serpin Peptidase Inhibitor, Clade D (Heparin Cofactor), Member 1 (SERPIND1))

Autre désignation: SERPIND1///Heparin Cofactor II (SERPIND1 Produits)

Synonymes: D22S673 Kit ELISA, HC2 Kit ELISA, HCF2 Kit ELISA, HCII Kit ELISA, HLS2 Kit ELISA, LS2 Kit ELISA, THPH10 Kit ELISA, serpind1 Kit ELISA, MGC53936 Kit ELISA, rls2var1 Kit ELISA, AA985900 Kit ELISA, AI303446 Kit ELISA, Hcf2 Kit ELISA, serpin family D member 1 Kit ELISA, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 L homeolog Kit ELISA, serpin peptidase inhibitor, clade D (heparin cofactor), member 1 Kit ELISA, serine (or cysteine) peptidase inhibitor, clade D, member 1 Kit ELISA, SERPIND1 Kit ELISA, serpind1.L Kit ELISA, Serpind1 Kit ELISA, serpind1 Kit ELISA

ID gène: 3053

UniProt: P05546