DIABLO Kit ELISA

N° du produit ABIN4884576

Détail du produit DIABLO Kit ELISA

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.1-30 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human SMAC ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detect human SMAC. Other species not determined yet.

Sensibilité: 100 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour DIABLO Kit ELISA (ABIN4884576)

Wiita, Hsu, Lu, Esensten, Wells: "Circulating proteolytic signatures of chemotherapy-induced cell death in humans discovered by N-terminal labeling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 21, pp. 7594-9, (2014) (PubMed).

Détail du antigène

Antigène: DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))

Autre désignation: SMAC (DIABLO Produits)

Synonymes: smac Kit ELISA, smac3 Kit ELISA, diablo-s Kit ELISA, 0610041G12Rik Kit ELISA, 1700006L01Rik Kit ELISA, AU040403 Kit ELISA, Smac Kit ELISA, DFNA64 Kit ELISA, SMAC Kit ELISA, DBO Kit ELISA, XSmac Kit ELISA, diablo, IAP-binding mitochondrial protein Kit ELISA, diablo IAP-binding mitochondrial protein Kit ELISA, diablo, IAP-binding mitochondrial protein L homeolog Kit ELISA, diablo Kit ELISA, Diablo Kit ELISA, DIABLO Kit ELISA, diablo.L Kit ELISA

Sujet: Gene Names: DIABLO SMAC
Protein names: Diablo homolog, mitochondrial (Direct IAP-binding protein with low pI) (Second mitochondria-derived activator of caspase) (Smac)

ID gène: 56616

UniProt: Q9NR28

Pathways: Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity