CEACAM1 Kit ELISA

N° du produit ABIN4885168

Détail du produit CEACAM1 Kit ELISA

Antigène: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human CEACAM-1 SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: CEACAM1 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 (CEACAM1))

Autre désignation: CEACAM-1 (CEACAM1 Produits)

Synonymes: BGP Kit ELISA, BGP1 Kit ELISA, BGPI Kit ELISA, CD66e Kit ELISA, CEA Kit ELISA, zeh0068 Kit ELISA, hm:zeh0068 Kit ELISA, CEACAM8 Kit ELISA, CEACAM1 Kit ELISA, MGC142545 Kit ELISA, BGPR Kit ELISA, Bgp Kit ELISA, CD66a Kit ELISA, Ccam1 Kit ELISA, Bgp1 Kit ELISA, C-CAM Kit ELISA, Cc1 Kit ELISA, Cea-1 Kit ELISA, Cea-7 Kit ELISA, Cea1 Kit ELISA, Cea7 Kit ELISA, Hv-2 Kit ELISA, Hv2 Kit ELISA, MHVR Kit ELISA, MHVR1 Kit ELISA, Mhv-1 Kit ELISA, bb-1 Kit ELISA, mCEA1 Kit ELISA, mmCGM1 Kit ELISA, mmCGM1a Kit ELISA, mmCGM2 Kit ELISA, carcinoembryonic antigen related cell adhesion molecule 1 Kit ELISA, carcinoembryonic antigen related cell adhesion molecule 5 Kit ELISA, carcinoembryonic antigen-related cell adhesion molecule 1 Kit ELISA, carcinoembryonic antigen-related cell adhesion molecule 6 Kit ELISA, CEACAM1 Kit ELISA, CEACAM5 Kit ELISA, ceacam1 Kit ELISA, Ceacam1 Kit ELISA, LOC101103343 Kit ELISA

ID gène: 634

UniProt: P13688