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IGFBP7 Kit ELISA

IGFBP7 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN4885255
  • Antigène Voir toutes IGFBP7 Kits ELISA
    IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
    Reactivité
    • 5
    • 2
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human IGFBP-rp1 (IGFBP-7) SpeedELISA Kit.
    Marque
    SpeedELISA
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Attributs du produit
    • Compressed workflow with only one wash step and three incubation steps
    • 3 hours total processing time
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Streptavidin-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Capture Antibody
    • HRP Conjugated Detection Antibody
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 70 μL of the Antibody Cocktail to each well.
    3. Incubate 10 minutes at RT.
    4. Add 50 μL of sample or standard to each well.
    5. Incubate 2 h at RT.
    6. Wash Wells
    7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    8. Incubate 30 min at RT.
    9. Add 50 μL of Stop Solution to each well.
    10. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
    Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
    Date de péremption
    6 months
  • Antigène Voir toutes IGFBP7 Kits ELISA
    IGFBP7 (Insulin-Like Growth Factor Binding Protein 7 (IGFBP7))
    Autre désignation
    IGFBP-Rp1 (IGFBP7 Produits)
    Synonymes
    zgc:85888 Kit ELISA, AGM Kit ELISA, FSTL2 Kit ELISA, IBP-7 Kit ELISA, IGFBP-7 Kit ELISA, IGFBP-7v Kit ELISA, IGFBPRP1 Kit ELISA, MAC25 Kit ELISA, PSF Kit ELISA, RAMSVPS Kit ELISA, TAF Kit ELISA, Fstl2 Kit ELISA, Mac25 Kit ELISA, insulin like growth factor binding protein 7 Kit ELISA, insulin-like growth factor binding protein 7 Kit ELISA, IGFBP7 Kit ELISA, igfbp7 Kit ELISA, Igfbp7 Kit ELISA
    ID gène
    3490
    UniProt
    Q16270
    Pathways
    Growth Factor Binding
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