RBP4 Kit ELISA

N° du produit ABIN4885324

Détail du produit RBP4 Kit ELISA

Antigène: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human RBP4 SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: RBP4 (Retinol Binding Protein 4, Plasma (RBP4))

Autre désignation: RBP4 (RBP4 Produits)

Synonymes: RDCCAS Kit ELISA, RBP Kit ELISA, RNBP Kit ELISA, rbp4 Kit ELISA, MGC54038 Kit ELISA, srbp Kit ELISA, PRBP Kit ELISA, RBPA Kit ELISA, Rbp-4 Kit ELISA, fb23c12 Kit ELISA, rbp Kit ELISA, wu:fb23c12 Kit ELISA, wu:fb58d04 Kit ELISA, wu:fb72b04 Kit ELISA, zgc:86911 Kit ELISA, retinol binding protein 4 Kit ELISA, renin binding protein Kit ELISA, retinol binding protein 4 S homeolog Kit ELISA, retinol binding protein 4 A, plasma Kit ELISA, retinol binding protein 4, plasma Kit ELISA, retinol binding protein 4 L homeolog Kit ELISA, RBP4 Kit ELISA, RENBP Kit ELISA, rbp4.S Kit ELISA, rbp4 Kit ELISA, Rbp4 Kit ELISA, RBP4A Kit ELISA, rbp4.L Kit ELISA

ID gène: 5950

UniProt: P02753

Pathways: Regulatory RNA Pathways, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response