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BAD Kit ELISA
BAD
Reactivité: Souris
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885429
Détail du produit BAD Kit ELISA
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Antigène
Voir toutes BAD Kits ELISA
BAD
(BCL2-Associated Agonist of Cell Death (BAD))
Reactivité
Toutes les réactivités sur BAD Kits ELISA
Souris
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Mouse BAD ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
The antibody pair provided in this kit recognizes Mouse BAD.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes BAD Kits ELISA
BAD
(BCL2-Associated Agonist of Cell Death (BAD))
Autre désignation
Bad (BAD Produits )
Synonymes
bad Kit ELISA, fa01b12 Kit ELISA, wu:fa01b12 Kit ELISA, wu:fa96d04 Kit ELISA, BAD Kit ELISA, BBC2 Kit ELISA, BCL2L8 Kit ELISA, AI325008 Kit ELISA, Bbc2 Kit ELISA, BCL2-associated agonist of cell death b Kit ELISA, BCL2 associated agonist of cell death Kit ELISA, BCL2-associated agonist of cell death Kit ELISA, badb Kit ELISA, BAD Kit ELISA, Bad Kit ELISA
ID gène
12015
UniProt
Q61337
Pathways
Signalisation MAPK , Signalisation PI3K-Akt , Signalisation RTK , Apoptose , Fc-epsilon Receptor Signaling Pathway , Positive Regulation of Peptide Hormone Secretion , Carbohydrate Homeostasis , Positive Regulation of Endopeptidase Activity , Regulation of Carbohydrate Metabolic Process , Hepatitis C , CXCR4-mediated Signaling Events
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