FAS Kit ELISA

N° du produit ABIN4885518

Détail du produit FAS Kit ELISA

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Mouse FAS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Mouse FAS.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Autre désignation: Fas (FAS Produits)

Synonymes: ALPS1A Kit ELISA, APO-1 Kit ELISA, APT1 Kit ELISA, CD95 Kit ELISA, FAS1 Kit ELISA, FASTM Kit ELISA, TNFRSF6 Kit ELISA, AI196731 Kit ELISA, APO1 Kit ELISA, TNFR6 Kit ELISA, Tnfrsf6 Kit ELISA, lpr Kit ELISA, Fas cell surface death receptor Kit ELISA, Fas (TNF receptor superfamily member 6) Kit ELISA, FAS Kit ELISA, Fas Kit ELISA, fas Kit ELISA

ID gène: 14102

UniProt: P25446

Pathways: Signalisation p53, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity