IGFBP5 Kit ELISA

N° du produit ABIN4885755

Détail du produit IGFBP5 Kit ELISA

Antigène: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Mouse IGFBP-5 SpeedELISA Kit.

Marque: SpeedELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Autre désignation: IGFBP-5 (IGFBP5 Produits)

Synonymes: IBP5 Kit ELISA, AI256729 Kit ELISA, AW208790 Kit ELISA, IGFBP-5 Kit ELISA, IGFBP-5P Kit ELISA, IGF-BP5 Kit ELISA, igfbp5 Kit ELISA, ibp5 Kit ELISA, IBP-5 Kit ELISA, xIGFBP-5 Kit ELISA, IGFBP5 Kit ELISA, igfbp5b Kit ELISA, zgc:165472 Kit ELISA, insulin like growth factor binding protein 5 Kit ELISA, insulin-like growth factor binding protein 5 Kit ELISA, insulin like growth factor binding protein 5 L homeolog Kit ELISA, insulin-like growth factor binding protein 5b Kit ELISA, insulin-like growth factor-binding protein 5 Kit ELISA, insulin-like growth factor binding protein 5a Kit ELISA, IGFBP5 Kit ELISA, Igfbp5 Kit ELISA, igfbp5.L Kit ELISA, igfbp5b Kit ELISA, igfbp5 Kit ELISA, LOC100194500 Kit ELISA, igfbp5a Kit ELISA

ID gène: 16011

UniProt: Q07079

Pathways: Signalisation WNT, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding