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ROBO1 Kit ELISA

ROBO1 Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4885894
  • Antigène Voir toutes ROBO1 Kits ELISA
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Reactivité
    • 3
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rat ROBO1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes rat ROBO1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes ROBO1 Kits ELISA
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Autre désignation
    ROBO1 (ROBO1 Produits)
    Synonymes
    DUTT1 Kit ELISA, SAX3 Kit ELISA, AW494633 Kit ELISA, AW742721 Kit ELISA, Gm310 Kit ELISA, ROBO1 Kit ELISA, dutt1 Kit ELISA, roundabout Kit ELISA, sax3 Kit ELISA, MGC122264 Kit ELISA, roundabout-1 Kit ELISA, roundabout guidance receptor 1 Kit ELISA, roundabout, axon guidance receptor, homolog 1 (Drosophila) Kit ELISA, roundabout guidance receptor 1 L homeolog Kit ELISA, ROBO1 Kit ELISA, Robo1 Kit ELISA, robo1 Kit ELISA, robo1.L Kit ELISA
    ID gène
    58946
    UniProt
    O55005
    Pathways
    Positive Regulation of Endopeptidase Activity
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