Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

JAK1 Kit ELISA

JAK1 Reactivité: Humain, Rat, Souris pThr246 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN4886154
  • Antigène Voir toutes JAK1 Kits ELISA
    JAK1 (Janus Kinase 1 (JAK1))
    Épitope
    pThr246, total
    Reactivité
    Humain, Rat, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-PRAS40 (T246) & Total PRAS40 ELISA Kit. This assay semi-quantitatively measures phophorylated PRAS40 (Thr246) & Total PRAS40 in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human JAK1 phosphorylated at site Tyrosine-1022 and total JAK1
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product JAK1 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Al-Maghrebi, Renno, Al-Somali, Botras, Qadhi: "Lutein modulates transcription dysregulation of adhesion molecules and spermatogenesis transcription factors induced by testicular ischemia reperfusion injury: it could be SAFE." dans: Naunyn-Schmiedeberg's archives of pharmacology, Vol. 389, Issue 5, pp. 539-51, (2017) (PubMed).

  • Antigène Voir toutes JAK1 Kits ELISA
    JAK1 (Janus Kinase 1 (JAK1))
    Autre désignation
    JAK1 (JAK1 Produits)
    Synonymes
    JAK1A Kit ELISA, JAK1B Kit ELISA, JTK3 Kit ELISA, AA960307 Kit ELISA, C130039L05Rik Kit ELISA, wu:fb93e10 Kit ELISA, jak1 Kit ELISA, Janus kinase 1 Kit ELISA, tyrosine-protein kinase JAK1 Kit ELISA, JAK1 Kit ELISA, Jak1 Kit ELISA, jak1 Kit ELISA, LOC100286690 Kit ELISA
    ID gène
    3716
    UniProt
    P23458
    Pathways
    Signalistation JAK/STAT, Signalisation RTK, Interferon-gamma Pathway, Hepatitis C, Toll-Like Receptors Cascades, Unfolded Protein Response
Vous êtes ici:
Support technique