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PKMYT1 Kit ELISA

PKMYT1 Reactivité: Humain pThr495 Colorimetric Sandwich ELISA Cell Culture Lysate
N° du produit ABIN4886252
  • Antigène Voir toutes PKMYT1 Kits ELISA
    PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
    Épitope
    pThr495, total
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phospho-PKMYT1 (Thr495) and Total PKMYT1 ELISA Kit. This assay semi-quantitatively measures phophorylated PKMYT1 (Thr495) and Total PKMYT1 in lysate samples.
    Type d'échantillon
    Cell Culture Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    This ELISA kit recognizes Human PKMYT1 phosphorylated at site Threonine-495 as well as total PKMYT1.
    Attributs du produit
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes PKMYT1 Kits ELISA
    PKMYT1 (Protein Kinase, Membrane Associated tyrosine/threonine 1 (PKMYT1))
    Autre désignation
    PKMYT1 (PKMYT1 Produits)
    Synonymes
    MYT1 Kit ELISA, 6230424P17 Kit ELISA, AW209059 Kit ELISA, Myt1 Kit ELISA, RGD1305434 Kit ELISA, myt1 Kit ELISA, protein kinase, membrane associated tyrosine/threonine 1 Kit ELISA, protein kinase, membrane associated tyrosine/threonine 1 S homeolog Kit ELISA, PKMYT1 Kit ELISA, pkmyt1 Kit ELISA, Pkmyt1 Kit ELISA, pkmyt1.S Kit ELISA
    ID gène
    9088
    UniProt
    Q99640
    Pathways
    Mitotic G1-G1/S Phases, M Phase
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