KIT Kit ELISA

N° du produit ABIN4886282

Détail du produit KIT Kit ELISA

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Épitope: pTyr

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phosphotyrosine SCFR ELISA Kit. This assay semi-quantitatively measures phosphotyrosine SCFR in lysate samples.

Type d'échantillon: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: The antibody pair provided in this kit recognizes Human Tyrosine-Phosphorylated-SCFR.

Attributs du produit:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Biotinylated Anti-Phosphotyrosine Antibody
• Stop Solution
• Assay Diluent(s)
• Positive Control Sample
• Lysis Buffer
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Autre désignation: SCF R (KIT Produits)

Synonymes: C-Kit Kit ELISA, CD117 Kit ELISA, PBT Kit ELISA, SCFR Kit ELISA, Bs Kit ELISA, Fdc Kit ELISA, Gsfsco1 Kit ELISA, Gsfsco5 Kit ELISA, Gsfsow3 Kit ELISA, SCO1 Kit ELISA, SCO5 Kit ELISA, SOW3 Kit ELISA, Ssm Kit ELISA, Tr-kit Kit ELISA, W Kit ELISA, c-KIT Kit ELISA, c-kit Kit ELISA, MGF Kit ELISA, KIT Kit ELISA, C-KIT Kit ELISA, kit Kit ELISA, receptor Kit ELISA, sparse Kit ELISA, Xkl-1 Kit ELISA, kit-a Kit ELISA, kit-b Kit ELISA, kl1-A Kit ELISA, krk1 Kit ELISA, pbt Kit ELISA, scfr Kit ELISA, KIT proto-oncogene receptor tyrosine kinase Kit ELISA, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog a Kit ELISA, KIT proto-oncogene receptor tyrosine kinase S homeolog Kit ELISA, KIT Kit ELISA, Kit Kit ELISA, kita Kit ELISA, kit Kit ELISA, kit.S Kit ELISA

ID gène: 3815

UniProt: P10721

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity