Vasopressin Kit ELISA

N° du produit ABIN4889783

Détail du produit Vasopressin Kit ELISA

Antigène: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1-1.000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: Human/Mouse/Rat Neurophysin-2 EIA Kit optimized for serum, plasma, and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat detect Neurophysin 2 and the propeptide Vasopressin-Neurophysin 2-copeptin.

Sensibilité: 1.4 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Préparation des réactifs:

Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Autre désignation: Neurophysin-2 (AVP Produits)

Synonymes: ADH Kit ELISA, ARVP Kit ELISA, AVP-NPII Kit ELISA, AVRP Kit ELISA, VP Kit ELISA, 2610201A18Rik Kit ELISA, Akr1a4 Kit ELISA, Vp Kit ELISA, Vsp Kit ELISA, DI Kit ELISA, Vas Kit ELISA, Adh Kit ELISA, Adh1a Kit ELISA, Adh1c Kit ELISA, adh Kit ELISA, arvp Kit ELISA, avrp Kit ELISA, avp-npii Kit ELISA, copeptin Kit ELISA, vasotocin Kit ELISA, arginine vasopressin Kit ELISA, aldo-keto reductase family 1, member A1 (aldehyde reductase) Kit ELISA, alcohol dehydrogenase 1 (class I) Kit ELISA, AVP Kit ELISA, Akr1a1 Kit ELISA, Avp Kit ELISA, Adh1 Kit ELISA, avp Kit ELISA

ID gène: 24221

UniProt: P01186

Pathways: cAMP Metabolic Process