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GTF2E1 Kit CLIA

GTF2E1 Reactivité: Humain Chemiluminescent Sandwich ELISA 41.15 pg/mL - 30000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
N° du produit ABIN492017
  • Antigène Tous les produits GTF2E1
    GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))
    Reactivité
    Humain
    Méthode de détection
    Chemiluminescent
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    41.15 pg/mL - 30000 pg/mL
    Seuil minimal de détection
    41.15 pg/mL
    Application
    ELISA
    Fonction
    The Chemiluminescent Immunoassay kit is designed for the in vitro sensitive quantitative measurement of FE in human serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité

    This assay has high sensitivity and excellent specificity for detection of Ferritin (FE).
    No significant cross-reactivity or interference between Ferritin (FE) and analogues was observed.

    Réactivité croisée (Details)
    No significant cross-reactivity or interference between Ferritin (FE) and analogues was observed.
    Sensibilité
    17.7 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate
    • Plate sealer for 96 wells
    • Standard
    • Standard Diluent
    • Assay Diluent A
    • Assay Diluent B
    • Standard
    • Detection Reagent A
    • Detection Reagent B
    • Substrate A
    • Substrate B
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Matériel non inclus
    • Luminometer capable of reading 96-well microplates with the following parameters: lag time 30.0s, read time 1.0 s/well .
    • Precision single or multi-channel pipettes and pipette tips with disposable tips.
    • Eppendorf Tubes for diluting samples.
    • Deionized or distilled water.
    • Absorbent paper for blotting the microtiter plate.
    • Container for Wash Solution
  • Indications d'application
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Commentaires

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Volume d'échantillon
    100 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    The microplate provided in this kit has been pre-coated with an antibody specific to Ferritin (FE). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Ferritin (FE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Ferritin (FE) level in the sample or standard.,
    Préparation des réactifs
    • Bring all kit components and samples to room temperature (18-25°C) before use.
    • Standard - Reconstitute the Standard with 1.0mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 10ng/mL. Please prepare 7 tubes containing 0.5mL Standard Diluent and produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.312ng/mL, 0.156ng/mL, and the last EP tubes with Standard Diluent is the blank as 0ng/mL.
    • Assay Diluent A and Assay Diluent B - Dilute 6mL of Assay Diluent A or B Concentrate(2×) with 6mL of deionized or distilled water to prepare 12 mL of Assay Diluent A or B. (In fact, more than 6mL Assay Diluent A and Assay Diluent B are contained in the bottles. Therefore, in every test, please precisely pipette required amount of Diluent and make double dilution in a new container. The prepared working dilution cannot be frozen.)
    • Detection Reagent A and Detection Reagent B - Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute to the working concentration with working Assay Diluent A or B, respectively (1:100).
    • Wash Solution - Dilute 20mL of Wash Solution concentrate (30×) with 580mL of deionized or distilled water to prepare 600 mL of Wash Solution (1×).
    • Substrate working Solution - Mix the substrate A and B by the ratio of 99:1 to make the substrate working solution. Mix thoroughly. For example, prepare 1,000µL Substrate working Solution with 990µL Substrate A + 10µL Substrate B.
    Note:
    • Making serial dilution in the wells directly is not permitted.
    • Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37°C directly.
    • Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10µL for once pipetting.
    • The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    • Prepare Substrate working Solution within 15 minutes before assay.
    • If crystals have formed in the Wash Solution concentrate (30×), warm to room temperature and mix gently until the crystals are completely dissolved.
    • Contaminated water or container for reagent preparation will influence the detection result.
    Prélèvement de l'échantillon
    Serum: Allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 20 minutes at approximately 1000 × g. Assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

    Plasma: Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g within 30 minutes of collection. Remove plasma and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

    Tissue Homogenates: The preparation of tissue homogenates will vary depending upon tissue type. For this assay, rinse tissues in ice-cold PBS (0.02 mol/L,pH 7.0-7.2) to remove excess blood thoroughly and weigh before homogenization. Mince the tissues to small pieces and homogenize them in 5-10 mL of PBS with a glass homogenizer on ice (Micro Tissue Grinders work, too). Sonicate the resulting suspension with an ultrasonic cell disrupter or subject it to two freeze-thaw cycles to further break the cell membranes. Centrifugate the homogenates for 5 minutes at 5000 × g. Remove the supernate and assay immediately or aliquot and store at -20°C

    Cell Culture Supernatant: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.

    Biological Fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20 °C or -80 °C for later use. Avoid repeated freeze/thaw cycles.
    Préparation de l'échantillon
    • We are only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
    • Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments. Sample should be diluted by 0.01mol/L PBS(PH=7.0-7.2).
    • If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
    • Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts from certain chemicals.
    • Due to the possibility of mismatching between antigen from other origin and antibody used in our kits (e.g.antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by our products.
    • Influenced by the factors including cell viability, cell number or sampling time, samples from cell culture supernatant may not be detected by the kit.
    • Fresh samples without long time storage is recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.
    Procédure de l'essai
    • 1. Determine wells for diluted standard, blank and sample. Prepare 7 wells for standard, 1 well for blank. Add 100 µL each of dilutions of standard (read Reagent Preparation), blank and samples into the appropriate wells. Cover with the Plate sealer. Incubate for 2 hours at 37 °C.
    • 2. Remove the liquid of each well, don't wash.
    • 3. Add 100 µL of Detection Reagent A working solution to each well. Incubate for 1 hour at 37 °C after covering it with the Plate sealer.
    • 4. Aspirate the solution and wash with 350 µL of 1× Wash Solution to each well using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher, and let it sit for 1~2 minutes. Remove the remaining liquid from all wells completely by snapping the plate onto absorbent paper. Repeat 3 times. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against absorbent paper.
    • 5. Add 100 µL of Detection Reagent B working solution to each well. Incubate for 30 minutes at 37 °C after covering it with the Plate sealer.
    • 6. Repeat the aspiration/wash process for five times as conducted in step 4.
    • 7. Add 100 µL of Substrate working Solution to each well. Cover with a new Plate sealer. Incubate for 5-10 minutes at 37 °C (Don't exceed 10 minutes). Protect from light.
    • 8. Measure the chemiluminescence signal in a microplate luminometer or as appropriate for the instrument used.
    Note:
    • 1. Assay preparation: Keep appropriate numbers of wells for 1 experiment and remove extra wells from microplate. Rest wells should be resealed and stored at -20 °C.
    • 2. Samples or reagents addition: Please use the freshly prepared Standard. Please carefully add samples to wells and mix gently to avoid foaming. Do not touch the well wall. For each step in the procedure, total dispensing time for addition of reagents or samples to the assay plate should not exceed 10 minutes. This will ensure equal elapsed time for each pipetting step, without interruption. To avoid cross-contamination, change pipette tips between additions of standards, samples, and reagents. Also, use separated reservoirs for each reagent.
    • 3. Incubation: To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. Once reagents are added to the well strips, DO NOT let the strips DRY at any time during the assay. Incubation time and temperature must be controlled.
    • 4. Washing: The wash procedure is critical. Complete removal of liquid at each step is essential for good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting and remove any drop of water and fingerprint on the bottom of the plate. Insufficient washing will result in poor precision and false elevated absorbance reading.
    • 5. For Substrate A and B, please protect it from light.
    • 6. Relative light units (RLUs) may differ from different luminometers.The Immunoassay was optimized using a Beijing Hamamatsu luminometer. Other instruments may require settings to be adjusted.
    • 7. Relative light units may vary within the 10 minute reading window.
    Calcul des résultats

    Average the duplicate readings for each standard, control, and samples and subtract the average zero standard relative light unit (RLU). Create a standard curve on log-log graph paper, with FE concentration on the y-axis and the RLU value on the x-axis. Draw the best fit straight line through the standard points and it can be determined by regression analysis. Using some plot software, such as curve expert 1.30, is also recommended. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
    In order to make the calculation easier, we plot the RLU value of the standard (X-axis) against the known concentration of the standard (Y-axis), although concentration is indeed the independent variable while RLU value is the dependent variable. Further, in this part, in order to help the customer perform the assay more visual, we provide the customer with the raw data (not the log of data). However, plotting log of the data to construct the curve will be recommended. The RLU values of the standard curve may vary according to the conditions of assay performance (e.g. operator, pipetting technique, washing technique or temperature effects). This curve is provided for demonstration only. The customers should establish their own standard curve for each test conducted.

    Précision du teste

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin (FE) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin (FE) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Conseil sur la manipulation
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Stock
    4 °C
    Stockage commentaire
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Date de péremption
    6 months
  • Antigène Tous les produits GTF2E1
    GTF2E1 (General Transcription Factor IIE, Polypeptide 1, alpha 56kDa (GTF2E1))
    Autre désignation
    FE (GTF2E1 Produits)
    Synonymes
    2610024P03Rik Kit CLIA, 56kDa Kit CLIA, AI303800 Kit CLIA, FE Kit CLIA, TFIIE-A Kit CLIA, DDBDRAFT_0168201 Kit CLIA, DDBDRAFT_0168202 Kit CLIA, DDBDRAFT_0231096 Kit CLIA, DDB_0168201 Kit CLIA, DDB_0168202 Kit CLIA, DDB_0231096 Kit CLIA, DDBDRAFT_0217176 Kit CLIA, DDBDRAFT_0217177 Kit CLIA, DDBDRAFT_0266401 Kit CLIA, DDB_0217176 Kit CLIA, DDB_0217177 Kit CLIA, DDB_0266401 Kit CLIA, TF2E1 Kit CLIA, si:ch211-194d6.2 Kit CLIA, gtf2e1 Kit CLIA, tfiiealpha Kit CLIA, general transcription factor II E, polypeptide 1 (alpha subunit) Kit CLIA, general transcription factor IIE subunit 1 Kit CLIA, transcription factor IIE Kit CLIA, general transcription factor IIE subunit 2 Kit CLIA, general transcription factor IIE, polypeptide 2, beta 34kDa Kit CLIA, general transcription factor IIE, polypeptide 1, alpha Kit CLIA, general transcription factor IIE subunit 1 L homeolog Kit CLIA, Gtf2e1 Kit CLIA, gtf2e1-1 Kit CLIA, gtf2e1-2 Kit CLIA, GTF2E2 Kit CLIA, GTF2E1 Kit CLIA, gtf2e1 Kit CLIA, gtf2e1.L Kit CLIA
    UniProt
    P02794
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