IL12B Kit ELISA

N° du produit ABIN4986929

Détail du produit IL12B Kit ELISA

Antigène: IL12B (Interleukin 12 beta (IL12B))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.02-1.0 ng/mL

Seuil minimal de détection: 0.02 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (citrate), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant Rat IL-12p40 Ligand

Sensibilité: 7 pg/mL

Matériel non inclus:

• Microplate reader.
• Pipettes and pipette tips.
• EP tube Deionized or distilled water.

Information d'application

Indications d'application: Detection Wavelength: 450 nm

Volume d'échantillon: 20 μL

Durée du test: 3 h

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IL12B (Interleukin 12 beta (IL12B))

Autre désignation: IL-12p40 (IL12B Produits)

Synonymes: CLMF Kit ELISA, CLMF2 Kit ELISA, IL-12B Kit ELISA, NKSF Kit ELISA, NKSF2 Kit ELISA, p40 Kit ELISA, Il-12b Kit ELISA, Il12p40 Kit ELISA, Il-12p40 Kit ELISA, Il12 Kit ELISA, IL-12p40 Kit ELISA, IL-12 Kit ELISA, IL-12 p40 Kit ELISA, il12b Kit ELISA, il12p40.b Kit ELISA, zgc:152789 Kit ELISA, IL-12.p40 Kit ELISA, IL12p40 Kit ELISA, IL12B Kit ELISA, CLMF p40 Kit ELISA, interleukin 12B Kit ELISA, interleukin 12b Kit ELISA, interleukin 12Ba Kit ELISA, IL12B Kit ELISA, Il12b Kit ELISA, il12ba Kit ELISA

Sujet: Interleukin 12(IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a pleiotropic cytokine originally identified in the medium of activated human B lymphoblastoid cell lines. IL-12 is produced by macrophages and B lymphocytes and has multiple effects on T-cells and NK cells, including stimulation of cytotoxic activity, proliferation, and promotion of Th1 development as well as IFNγ and TNF production. IL-12 is a disulfidelinked, 70 kDa(p70) heterodimeric glycoprotein composed of a 40 kDA (p40) subunit and a 35 kDa (p35) subunit. The p40 and p35 subunits by themselves have no IL-12 activity, the p40 dimer has been shown to bind the IL-12 receptor and to be an IL-12 antagonist. Free p35 has not been detected in supernatant solutions of cultured cells expressing only p35 or both p35 and p40 mRNAs. In contrast, p40 is secreted in excess of IL-12 in cells expressing both p35 and p40 mRNAs. The p40 subunit of IL-12 has been shown to have extensive amino acid sequence homology to the extracellular domain of the human IL- 6 receptor while the p35 subunit shows distant but significant sequence similarity to IL-6, G-CSF, and chicken MGF. These observations have led to the suggestion that IL -12 might have evolved from a cytokine/soluble receptor complex. Human and mouse IL-12 share 70 % and 60 % amino acid sequence homology in their p40 and p35 subunits, respectively. IL-12 apparently shows species specificity with human IL-12 reportedly showing minimal activity in the murine system.

Pathways: Signalistation JAK/STAT, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation