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Fc Receptor-Like 3 (FCRL3) Kit ELISA Kit ELISA

FCRL3 Reactivité: Humain Colorimetric Sandwich ELISA 0.156-10 ng/mL Plasma
N° du produit ABIN4993442
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96 tests
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  • Antigène
    Méthode de détection
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156-10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of FCRL3 in human serum, plasma and other biological fluids
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    This kit recognizes natural and recombinantHumanFCRL3. No significant cross-reactivity or interference between HumanFCRL3 and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between HumanFCRL3 and all the analogues.
    0.094 ng/mL
    Micro ELISA Plate
    Reference Standard
    Concentrated Biotinylated Detection Ab
    Concentrated HRP Conjugate
    Reference Standard & Sample Diluent
    Biotinylated Detection Ab Diluent
    HRP Conjugate Diluent
    Concentrated Wash Buffer (25×)
    Substrate Reagent
    Stop Solutio
    Plate Sealer
    Product Description
    Certificate of Analysis
    Matériel non inclus
    Microplate reader with 450nm wavelength filter
    High-precision transferpettor, EP tubes and disposable pipette tips
    37℃ Incubator
    Deionized or distilled water
    Absorbent paper
    Loading slot for Wash Buffer
  • Commentaires

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Volume d'échantillon
    100 µL
    This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FCRL3. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FCRL3and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FCRL3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of FCRL3.You can calculate the concentration of FCRL3in the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 4
    For Research Use only
  • Conseil sur la manipulation
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    4 °C/-20 °C
    Stockage commentaire
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Antigène
    Autre désignation
    Fc receptor-like protein 3 (FCRL3) (FCRL3 ELISA Kit Extrait)
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