RARS Kit ELISA

N° du produit ABIN4993652

Détail du produit RARS Kit ELISA

Antigène: RARS (Arginyl-tRNA Synthetase (RARS))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.781-50 ng/mL

Seuil minimal de détection: 0.781 ng/mL

Application: ELISA

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RARS in Mouse serum, plasma and other biological fluids

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This kit recognizes natural and recombinantMouseRARS. No significant cross-reactivity or interference between MouseRARS and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between MouseRARS and all the analogues.

Sensibilité: 0.469 ng/mL

Ingrédients: Micro ELISA Plate
Reference Standard
Concentrated Biotinylated Detection Ab
Concentrated HRP Conjugate
Reference Standard & Sample Diluent
Biotinylated Detection Ab Diluent
HRP Conjugate Diluent
Concentrated Wash Buffer (25×)
Substrate Reagent
Stop Solutio
Plate Sealer
Product Description
Certificate of Analysis

Matériel non inclus: Microplate reader with 450nm wavelength filter
High-precision transferpettor, EP tubes and disposable pipette tips
37℃ Incubator
Deionized or distilled water
Absorbent paper
Loading slot for Wash Buffer

Information d'application

Commentaires:

Information on standard material:
The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

Information on reagents:
Reagents include 1 M SO₂. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

Information on antibodies:
The provided antibodies and their host vary in different kits. All antibodies are affinity purified

The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Volume d'échantillon: 100 µL

Plaque: Pre-coated

Protocole: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to RARS. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for RARSand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain RARS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of RARS.You can calculate the concentration of RARSin the samples by comparing the OD of the samples to the standard curve. 6th Edition, revised in June, 2015 www.elabscience.com 4

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.

Stock: 4 °C/-20 °C

Stockage commentaire: The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.

Détail du antigène

Antigène: RARS (Arginyl-tRNA Synthetase (RARS))

Autre désignation: Arginyl tRNA Synthetase (RARS) (RARS Produits)

Synonymes: ArgRS Kit ELISA, CG9020 Kit ELISA, Dmel\\CG9020 Kit ELISA, RRS Kit ELISA, wu:fa96g09 Kit ELISA, zgc:56050 Kit ELISA, zgc:86730 Kit ELISA, RARS Kit ELISA, xcc-b100_4045 Kit ELISA, F28G11.14 Kit ELISA, F28G11_14 Kit ELISA, ECK1877 Kit ELISA, JW1865 Kit ELISA, lov Kit ELISA, 2610011N19Rik Kit ELISA, 2610037E21Rik Kit ELISA, AL033339 Kit ELISA, AW985894 Kit ELISA, DALRD1 Kit ELISA, ARGRS Kit ELISA, Arginyl-tRNA synthetase Kit ELISA, arginyl-tRNA synthetase Kit ELISA, hypothetical protein Kit ELISA, arginyL-tRNA synthetase Kit ELISA, Arginyl-tRNA synthetase, class Ic Kit ELISA, arginine--tRNA ligase Kit ELISA, ArgRS Kit ELISA, rars Kit ELISA, RARS Kit ELISA, ZMO0843 Kit ELISA, TP01_1024 Kit ELISA, Mmc1_0704 Kit ELISA, Oter_2566 Kit ELISA, argS Kit ELISA, TSTA_030410 Kit ELISA, PAAG_11057 Kit ELISA, MCYG_01632 Kit ELISA, Arch_0351 Kit ELISA, VDIS_RS05935 Kit ELISA, LOC100281742 Kit ELISA, Calkr_0529 Kit ELISA, Palpr_2492 Kit ELISA, Mspyr1_17280 Kit ELISA, AT1G66530 Kit ELISA, UCYN_05320 Kit ELISA, Rars Kit ELISA