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CXCL1 Kit ELISA

CXCL1 Reactivité: Rat Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Plasma, Serum
N° du produit ABIN4993683
  • Antigène Voir toutes CXCL1 Kits ELISA
    CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
    Reactivité
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.63 pg/mL - 1000 pg/mL
    Seuil minimal de détection
    15.63 pg/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.
    Type d'échantillon
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This kit recognizes natural and recombinantRatSDF-1α. No significant cross-reactivity or interference between RatSDF-1α and analogues was observed. Note: Limited by existing techniques, cross reaction may still exist, as it is impossible for us to complete the cross-reactivity detection between RatSDF-1α and all the analogues.
    Sensibilité
    9.38 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Reference Standard & Sample Diluent
    • Biotinylated Detection Antibody (100 x concentrate)
    • HRP Conjugate (100 x concentrate)
    • Biotinylated Detection Antibody Diluent
    • HRP Conjugate Diluent
    • Substrate Reagent
    • Stop Solution
    • Wash Buffer (25 x concentrate)
    • Instruction manual
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  • Commentaires

    Information on standard material:
    The formulation of the standard is 0.01 M PBS. The standard contains additives (1 % BSA).

    Information on reagents:
    Reagents include 1 M SO2. Azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials are not used.

    Information on antibodies:
    The provided antibodies and their host vary in different kits. All antibodies are affinity purified

    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

    Volume d'échantillon
    100 µL
    Plaque
    Pre-coated
    Protocole
    1. Add 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.
    2. Remove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.
    3. Aspirate and wash 3 times.
    4. Add 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.
    5. Aspirate and wash 5 times.
    6. Add 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.
    7. Add 50 µL Stop Solution. Read at 450 nm immediately.
    8. Calculation of results.
    Préparation des réactifs
    1. Bring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.
    2. Wash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved
    3. Standard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard &Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 1000 pg/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 1000, 500, 250, 125, 62.5, 31.25, 15.63, 0 pg/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard & Sample Diluent to each tube. Pipette 500 μLof the 1000 pg/mL working solution to the first tube and mix up to produce a 500 pg/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.
    4. Biotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.
    5. Concentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    All the reagents in the kit should be stored according to the labels on vials. Unused wells should be returned to the foil pouch with the desiccant pack and resealed along entire edge of zip-seal. Substrate Reagent shouldn't be kept at -20 °C (Check!). Exposure of reagents to strong light should be avoided in the process of incubation and storage. All the taps of reagents should be tightened to prevent evaporation and microbial contamination. If not to store reagents according to above suggestions, erroneous results may occur.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the conditions since the kit is received.
  • Antigène Voir toutes CXCL1 Kits ELISA
    CXCL1 (Chemokine (C-X-C Motif) Ligand 1 (Melanoma Growth Stimulating Activity, Alpha) (CXCL1))
    Autre désignation
    Stromal Cell Derived Factor 1alpha (CXCL1 Produits)
    Synonymes
    FSP Kit ELISA, GRO1 Kit ELISA, GROa Kit ELISA, MGSA Kit ELISA, MGSA-a Kit ELISA, NAP-3 Kit ELISA, SCYB1 Kit ELISA, CINC-1 Kit ELISA, Gro1 Kit ELISA, CXCL1 Kit ELISA, Sdf1 Kit ELISA, Fsp Kit ELISA, KC Kit ELISA, Mgsa Kit ELISA, N51 Kit ELISA, Scyb1 Kit ELISA, gro Kit ELISA, GRO Kit ELISA, Gro Kit ELISA, C-X-C motif chemokine ligand 1 Kit ELISA, chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha) Kit ELISA, C-X-C motif chemokine ligand 12 Kit ELISA, chemokine (C-X-C motif) ligand 1 Kit ELISA, CXCL1 Kit ELISA, Cxcl1 Kit ELISA, Cxcl12 Kit ELISA, GRO1 Kit ELISA
    Pathways
    Autophagy
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