TNF alpha Kit ELISA

N° du produit ABIN5026952

Détail du produit TNF alpha Kit ELISA

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Human TNFAlpha concentrations in serum, plasma and other biological fluids.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Sensibilité: < 9.4 pg/mL

Ingrédients: plate, standard, diluent, antibodies

Matériel non inclus: pipettes, tubes, reader, incubator

Information d'application

Volume d'échantillon: 100 μL

Durée du test: 3 - 4 h

Plaque: Pre-coated

Protocole: This assay employs an antibody specific for Anti-human TNFAlpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFAlpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFAlpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFAlpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: protect your eyes

Stock: 4 °C

Détail du antigène

Antigène: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Autre désignation: TNFalpha (Tumor Necrosis Factor Alpha) (TNF alpha Produits)

Synonymes: DIF Kit ELISA, TNF-alpha Kit ELISA, TNFA Kit ELISA, TNFSF2 Kit ELISA, RATTNF Kit ELISA, Tnfa Kit ELISA, tnf Kit ELISA, TNF-a Kit ELISA, TNFalpha Kit ELISA, Tnfsf1a Kit ELISA, TNFa Kit ELISA, cTNF Kit ELISA, Tnf-alpha Kit ELISA, tnfa-like Kit ELISA, TNF-ALPHA Kit ELISA, dif Kit ELISA, tnfa Kit ELISA, xtnf Kit ELISA, tnfsf2 Kit ELISA, tnf-alpha Kit ELISA, Cachectin Kit ELISA, tumor necrosis factor Kit ELISA, tumor necrosis factor b (TNF superfamily, member 2) Kit ELISA, tumor necrosis factor alpha Kit ELISA, tumor necrosis factor a (TNF superfamily, member 2) Kit ELISA, TNF Kit ELISA, Tnf Kit ELISA, tnf Kit ELISA, tnfb Kit ELISA, tnf-alpha Kit ELISA, LOC103694380 Kit ELISA, tnfa Kit ELISA

Pathways: Signalisation NF-kappaB, Apoptose, Caspase Cascade in Apoptosis, Signalisation TLR, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome